用辣根过氧化酶检测其通透性。
本发明也公开了用该细胞系制备单克隆抗体、抗体提纯方法及该抗体的辣根过氧化酶标记法。
The invention also discloses preparation of the monoclonal antibody by using the cell line, a method for purifying the antibody and a horseradish peroxidase labeling method for the antibody.
通过固定辣根过氧化酶（HRP）标记CA19-9抗体在纳米金修饰的碳糊电极制备了CA19-9安培免疫传感器。
A novel immunosensor for the determination of carcinoma antigen-19-9(CA19-9) is made by immobilization of horseradish peroxidase(HRP) labeled CA19-9 antibody on colloidal gold carbon paste electrodes.
方法采用方阵滴定法，比较4种封闭液、不同稀释度的捕获抗体、检测抗体和辣根过氧化酶标记链菌素对检测结果的影响，确定ELISA法的最佳实验条件。
Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test.
方法采用方阵滴定法，比较4种封闭液、不同稀释度的捕获抗体、检测抗体和辣根过氧化酶标记链菌素对检测结果的影响，确定ELISA法的最佳实验条件。
Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test.
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