本发明公开了一种重组犬2型腺病毒转移载体、其构建方法及其用途。
The present invention discloses a recombinant canine adenovirus-2 transfer vector, a method for constructing the recombinant canine adenovirus-2 transfer vector and an applications thereof.
基因转移载体是基因治疗的重要组成部分，包括病毒载体和非病毒载体。
Gene transfer vector is the important ingredient in gene therapy, which includes virus vector and non-virus vector.
腺病毒作为一种重要的基因转移载体，在应用中存在着靶向性差的问题。
As an important gene transfer vector, adenovirus has the deficiency of poor targeting.
基因转移载体是基因治疗的重要组成部分，包括病毒载体和非病毒载体。
Gene transfer vector is the important ingredient in gene therapy, which includes virus vector and nan-vires vector.
本发明还涉及人源化改造的基因突变技 术和去除GFP的腺病毒转移载体及去除GFP的同源重组腺病毒载体。
The invention also relates to a human reforming gene mutation technique, adenovirus transition carrier removing GFP and homologous recombinant adenovirus carrier.
另外，杆状病毒是非复制型载体，能高效表达目的蛋白，其作为基因转移载体在基因治疗中乙显示出良好的应用前景。
Additionally, baculovirus has been a new gene transfer vector used in gene therapy due to its advantages of the extremely high expression of foreign genes and being non-reproduce vector.
“pheromone”（信息素）一词来源于希腊语的“pherein”(转移，传递)和“hormone”（兴奋，激素），表示一种“兴奋的载体”。
The word "pheromone" comes from the Greek words pherein and hormone, meaning "excitement carrier".
知识的价值由活劳动价值、知识产品的载体价值和知识产品中的转移价值构成。
Knowledge value is composed of human labor value, carrier value and transformation value of knowledge product.
逆转录病毒介导的基因转移技术要过渡到临床应用，主要解决如何使病毒载体具有高滴度而不具有复制活性。
In order to use retroviral-mediated gene transfer technology in clinical application, retroviral vector must be of high titer and free of detectable replication-competent retroviruses.
重组病毒载体因其自然感染途径而成为有效的基因转移手段。
Recombinant viral vectors have become effective tools for gene transfer by means of their natural infective ways.
通过改变循环移动载体膜生物反应器的结构尺寸，研究了反应器内氧转移系数的变化。
Changes of oxygen transfer coefficient were studied in a test of Circulated Moving Carrier Bio-membrane Reactor (CMCBR) by changing the design and size of the CMCBR.
目的：构建CD 147反义rna表达质粒载体，探索治疗骨肉瘤侵袭和转移的新方法。
AIM: to construct eukaryotic antisense RNA expression vector of CD147 and probe into a new method to treat invasion and transfer of osteosarcoma.
主要研究了碳纤维复合材料转移法金属化载体膜的制备。
The preparation of the carrier film which is used on CFRP metalizing by transfer is studied in this paper.
将外源的基因导入生物细胞内必须借助一定的技术方法或载体，目前基因转移的方法分为生物学方法、物理方法和化学方法。
The foreign gene into the biological cells must use a certain technology or approach vector, the current methods of gene transfer into biological methods, methods of physical and chemical methods.
采用相转移催化法以四丁基氯化铵为催化剂合成了端基含磺胺嘧啶的聚乳酸，将肿瘤导向基团引入了可生物降解药物载体。
Sulfadiazine-polylactide, a tumor targeting drug carrier, was successfully synthesized by the phase transfer catalysis(PTC)with tetrabutyl ammonium chloride hydroxide as catalyst.
试验结果表明，由于载体改性，使催化剂的抗硫性能得到明显改善，并且具备一定的硫转移能力。
The experiment result showed that the sulfur resistance performance of the modified promotor was obviously improved, and which also possessed certain sulfur transfer ability.
四氢叶酸（THF）辅酶是生物合成与细胞代谢的过程中一碳单元转移的载体。
Tetrahydrofolate(THF) cofactor is the carrier of one-carbon unit on biosynthesis and cell metabolize.
实际上，价值的大部分不是变小或消失，而转移了，随着人们的利用转移到其他文献形式或信息载体之中了。
In fact most of the value does not become small or lost but is transferred to other documental forms or information carriers with people's use.
众所周知，工业园是承接产业转移、加速产业集聚、培育产业集聚的主要空间载体。
As is known, industrial parks are the main carriers which carry on the industrial transfer and speed up the industrial concentration.
以跨国公司为载体的国际产业转移掀起新的浪潮。
The international industry taking trans-corporation as carrier has set off a new tide.
胡同文化会转移、分解、传承到新的载体上。
Alley cultural shift, decomposition, transmission to a new carrier.
在细胞生物学中用嘌罗霉素来选择用载体转化能表达嘌呤霉素- N -乙酰基转移酶类的哺乳动物细胞系。
Puromycin is used in cell biology to select mammalian cell lines that have been transformed by vectors that express puromycin-N-acetyl-transferase.
目的构建含小鼠白细胞介素12双亚基及新霉素磷酸转移酶基因多顺反子逆转录病毒载体，并观察其在小鼠肝癌细胞中的表达。
Objective to construct a polycistronic retroviral vector containing both subunits cDNA of murine IL 12 and NeoR gene and investigate their expressions in murine hepatoma cells.
对角膜内皮基因转移技术和载体的研究现状，及未来载体的研究方向进行综述。
The review summarized the current studies of gene transfer to corneal endothelium and vectors that are been using. In addition, the review indicates the study prospect for future vectors.
研究显示恩镰孢菌素是一个离子载体，最近，它们对酰基辅酶A胆固醇转移酶的效应、杀线虫活性和选择性的抗肿瘤活性得到了更多的关注。
The enniatins have been shown to act as ionophores. Recently their effects on acyl-CoA cholesterol transferase, as nematocides and the selectivity of their antitumor action have received more focus.
目的构建人胰岛素样生长因子- 1的逆转录表达载体，建立逆病毒介导的IGF - 1基因转移系统。
Objective to construct a retroviral mediated expression system of human insulin-like growth factor-1 (IGF-1), and determine whether bone marrow stromal cells (BMSCs) can be infected by retrovirus.
随着对病毒载体的不断改造完善，提高其基因转移效率和安全性，病毒载体将在基因治疗中继续发挥重要作用。
With the increasing reform and consummation of virus vector, it can play its important role continuously in gene therapy by promoting its efficacy and safty in gene transfer.
随着对病毒载体的不断改造完善，提高其基因转移效率和安全性，病毒载体将在基因治疗中继续发挥重要作用。
With the increasing reform and consummation of virus vector, it can play its important role continuously in gene therapy by promoting its efficacy and safty in gene transfer.
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