目的研究MTS1基因arf启动子基础转录调控区的转录激活及其与E2 F1转录因子之间的关系。
Objective to study the effect of E2F1 transcription factor on the transcriptional activity of basic transcriptional regulatory region in the ARF promoter of MTS1 gene.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
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