建立了筛选质粒表达文库和克隆免疫原基因的技术方法。
We established the techniques of screening the plasmid expression library and cloning the immunogen genes.
以肺腺癌细胞系a2为抗原进行4轮筛选，得到富集的次级噬菌体表达文库。
After 4 rounds of screening with lung adenocarcinoma cell line A2 as antigen, an enriched secondary phage display library was obtained.
一个包含可表达为蛋白质的基因的文库称为cDNA表达文库，它可以用来筛选结合特定抗体的蛋白。
A library containing the genes that could be expressed as proteins would be referred to as a cDNA expression library, which could be screened for a protein that binds a particular antibody.
用吸附后的血清通过菌落原位免疫杂交筛选该基因组表达文库，从3000个菌落中获得了12个阳性克隆。
The genomic expression library was probed used the adsorbed-pooled sera, and 12 positive clones were obtained from 3000 postulating colonies by immunological hybridization.
用吸附后的血清通过菌落原位免疫杂交筛选该基因组表达文库，从3000个菌落中获得了12个阳性克隆。
The genomic expression library was probed used the adsorbed-pooled sera, and 12 positive clones were obtained from 3000 postulating colonies by immunological hybridization.
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