目的：探讨大肠杆菌嘌呤核苷磷酸化酶自杀基因对胃癌细胞MKN-45的抑制及杀伤作用，并证实旁观者效应。
Objective To study the effects E. coli purine nucleoside phosphorylase suicide gene on human gastric cancer MKN-45 cells as well as the bystander effect.
结论KDR基因启动子可调控双自杀基因系统选择性杀伤表达KDR胃癌细胞及血管内皮细胞。
Conclusions the double suicide gene driven by KDR promoter has specific killing effect on KDR-expressing gastric tumor cells and venous endothelial cells in vitro.
结果单、双自杀基因均在GLC- 82细胞中稳定表达，双基因转染组对细胞增殖的杀伤及旁杀伤效应高于单基因组。
Results Double and single suicide gene transfer were both stably expressed in GLC-82 cells. The cytotoxic effects of co-expressed TK-CD genes were superior than that of the single gene.
结果单、双自杀基因均在GLC- 82细胞中稳定表达，双基因转染组对细胞增殖的杀伤及旁杀伤效应高于单基因组。
Results Double and single suicide gene transfer were both stably expressed in GLC-82 cells. The cytotoxic effects of co-expressed TK-CD genes were superior than that of the single gene.
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