目的：分离培养人脂肪基质干细胞，鉴定其表型。
AIM: To isolate and culture human adipose tissue derived stem cells, and identify their phenotype.
免疫荧光法鉴定脂肪基质干细胞表达CD13和CD44。
The adipose stem cells expressed CD13 and CD44 through immunofluorescence.
在微载体悬浮培养条件下，脂肪基质干细胞生长更为旺盛，细胞产量更高，优于常规静止培养。
Under the condition of microcarrier suspension culture, the adipose derived stem cells grew more productively, and the production of cell was higher, which were superior to those under static culture.
资料综合：脂肪组织来源干细胞与同样起源于中胚层的骨髓基质细胞不仅具有非常相似的生物学特性，而且在细胞表面标志谱的表达方面也非常相近。
DATA SYNTHESIS: There was similar bionomics of adipose tissue-derived stem cells and bone mesenchymal cells, and the cell-surface marker spectrum was also very similar.
结论：釉基质蛋白可作为一种成骨诱导剂，能有效增强大鼠脂肪源性干细胞体外增殖活性并诱导其成骨分化。
Conclusion: Enamel matrix proteins may be used as an osteoinductive agent, due its enhancing effect on adipose-derived stem cells proliferation and osteogenic differentiation.
骨髓基质干细胞是一种由骨髓中分离获得的具有多种分化潜能的间质干细胞。在体外培养条件下，它可以分化为成骨细胞、软骨细胞、脂肪细胞、甚至于成肌细胞。
Bone marrow stromal stem cells isolated from bone marrow are the special mesenchymal stem cells, which can be differentiated in culture into osteoblasts, chondrocytes, adipocytes, and even myoblasts.
骨髓基质干细胞是一种由骨髓中分离获得的具有多种分化潜能的间质干细胞。在体外培养条件下，它可以分化为成骨细胞、软骨细胞、脂肪细胞、甚至于成肌细胞。
Bone marrow stromal stem cells isolated from bone marrow are the special mesenchymal stem cells, which can be differentiated in culture into osteoblasts, chondrocytes, adipocytes, and even myoblasts.
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