化学修饰实验发现:巯基、胍基、氨基、羧基和吲哚基可能参与酶活性中心的组成。
Chemical modification studies showed that the sulfhydryl, guanido, amino, carboxyl and indolyl groups may participate in the active center of the enzyme.
目的观察磷酸盐对人胎盘碱性磷酸酶(HPAP)胍变性的影响,为酶活性部位的柔性理论提供证据。
Objective To study the effects of phosphate on guanidine denaturation of human placental alkaline phosphatase (HPAP) and provide a theoretical basis for flexibility hypothesis of enzyme active sites.
目的观测人胎盘碱性磷酸酶(HPAP)在盐酸胍变性和复性过程中构象与活力的变化,探讨其折叠机制。
Objective To examine the changes of conformation and activity of human placental alkaline phosphatase(HPAP) in denaturation and renaturation of guanidine chloride, and to study its folding mechanism.
用荧光相图法分别研究了脲和盐酸胍诱导牛肝过氧化氢酶去折叠的过程。
The unfolding of bovine liver catalase induced by urea or guanidine hydrochloride (GuHCl) has been studied by "phase diagram" method of fluorescence.
本文利用荧光、紫外差光谱研究了根霉葡萄糖淀粉酶在盐酸胍变性后的复性、复活动力学。
The kinetics of the renaturation and reactivation of glucoamylase denatured by guanidine HCl was determined by means of the UV differential absorbance measurement and fluorescence emission spectra.
在高浓度盐酸胍下酶失活主要是因为酶二聚体迅速解离成单亚基的过程和单亚基构象逐渐变化的缓慢过程。
The inactivation of aminoacylase in guanidine solution was caused by rapid dissociation of dimer into monomer which was then slowly changed into a stable conformation.
目的:探讨诱导型一氧化氮合酶抑制剂氨基胍对大鼠移植胰腺的保护作用。
Objective: to investigate the effect of inducible nitric oxide synthase inhibitor aminoguanidine on transplanted pancreas in rats.
目的:探讨诱导型一氧化氮合酶抑制剂氨基胍对大鼠移植胰腺的保护作用。
Objective: to investigate the effect of inducible nitric oxide synthase inhibitor aminoguanidine on transplanted pancreas in rats.
应用推荐