方法：采用聚合酶链式反应(PCR)。
采用逆转录聚合酶链式反应(RT - PCR)检测病毒。
Viral detection by reverse transcription polymerase chain reaction (RT-PCR) assay, and.
聚合酶链式反应仪(PcR仪)是分子生物学的基本仪器。
The polymerase chain reaction (PCR) machine is a basic instrument in molecule biology.
基因表达采用逆转录-聚合酶链式反应（RT-PCR）方法。
The mRNA was detected by using reverse transcription-polymerase chain reaction analyses(RT-PCR);
运用逆转录聚合酶链式反应测定心肌细胞内病毒相关基因的表达。
RT- PCR assays were used to determine expression of CVB_3 - associated genes in cardiomyocytes.
目的探讨聚合酶链式反应变性及退火温度与产物假阴性之间的关系。
Objective to explore the relationship of temperatures for denaturalization and annealing to the false negative PCR result.
例如，聚合酶链式反应(PCR)，这种DNA入扩增方法因在0。
Polymerase1 chain reaction (PCR), for example, the DNA-amplifying method used most famously in the 0.
介绍了分子生物学前沿技术聚合酶链式反应(PCR)的原理、方法及应用。
The principle, method and application of Polymerase Chain Reaction (PCR) was introduced in this paper.
聚合酶链式反应可使DNA自我复制，产生大量的DNA样品可供科学家进行研究。
Polymerase helps coax DNA to replicate itself, leaving researchers with large numbers of samples to work with.
聚合酶链式反应（PCR）在生命科学研究及诸多相关领域已经得到了广泛应用。
Polymerase chain reaction(PCR) has been widely applied in life science and other related fields.
实验室鉴定方法包括聚合酶链式反应、限制酶片段长度多态性分析以及生物芯片技术等。
In laboratories, the methods mainly include polymerase chain reaction, restriction fragment length polymorphism, and biochip technology. The methods mentioned above all have their merits and defects.
实验室鉴定方法包括聚合酶链式反应、限制酶片段长度多态性分析以及生物芯片技术等。
In laboratories, the methods mainly include polymerase chain reaction, restriction fragment length polymorphism, and biochip technology.
TED大会提到，Muliis发明了聚合酶链式反应技术的这一成就看起来可以盖过其他一些事。
Ted mentions that Mullis invented PCR and surfs and seems to skim over a few other things.
方法提取肿瘤组织总rna，用反转录聚合酶链式反应(RT - pcr)法进行检测。
Methods Total RNAs of tumor tissues were extracted and were detected by RT-PCR.
目的评估用逆转录聚合酶链式反应(RT PCR)方法检测胃癌腹腔微转移的临床病理意义。
Objective to evaluate the clinicopathologic significance of the detection of peritoneal micrometastases in gastric cancer by reverse transcriptase-polymerase chain reaction (RT-PCR).
聚合酶链式反应(PCR)技术已在分子生物学、基因测序、医学诊断等方面得到广泛的应用。
Polymerase chain reaction (PCR) technology has been successfully used in clinic diagnostic and modern molecular biology for gene analyzing.
“聚合酶链式反应”的简称，一种可在血液或骨髓中检测到癌细胞标志物存在的灵敏的实验室试验。
PCR the short name for "polymerase chain reaction," a sensitive lab test that can measure the presence of cancer cell-markers in the blood or marrow.
目的运用依赖随机化末端连接物聚合酶链式反应(RDPCR)技术检测环境中混合污染物的DNA损伤作用。
Objective To detect DNA lesions induced by complex pollutants existing in environment using the technique of randomized terminal linker, dependent PCR (RDPCR).
首先看看他的成就：他发明了一项叫做聚合酶链式反应的技术，这项技术对于生物学的意义就如同晶体管对于计算机的意义。
First his accomplishments: he invented a process called PCR, which is every bit as important to biology as the transistor is to computers.
本发明进一步提供用作分子探针或聚合酶链式反应(PCR)扩增引物的核酸分子，即合成的或天然的寡聚核苷酸。
The present invention further provides nucleic acid molecules for use as molecular probes, or as primers for polymerase chain reaction (PCR) amplification, i. e. synthetic or natural oligonucleotides.
检测橘蚜携带CTV的分子生物学反转录-聚合酶链式反应技术已建立，并已应用于检测橘蚜等蚜虫的单虫带毒情况。
Reverse transcription polymerase chain reaction technique for the detection of CTV in a single aphid or aphids has been developed.
本研究利用聚合酶链式反应技术，成功地克隆了枯草芽孢杆菌缺陷型原噬菌体PBSX阻遏基因及其温度敏感型等位基因。
The repressor gene and its allele-a temperature sensitive mutant have been cloned from the defective prophage PBSX of Bacillus subtilis by means of PCR technique.
P CR芯片是进行DNA聚合酶链式反应的芯片，温控部分的搭构是决定整个PCR芯片性能、尺寸、集成化的重要组成部分。
PCR-chip is a function biochip for DNA polymerase chain reaction. The temperature control section is so important that it decides the performance, dimension, integration of the whole chip.
血清学检测是针对麻风杆菌特异性式碳酸糖酯抗体进行，此外聚合酶链式反应（PCR）也可用于检测，但对于早期诊断并不可靠。
Serology aimed at M leprae-specific phenolic glycolipidantibodies and polymerase chain reaction may also be used but fail to reliablydetect early disease.
方法收集160例临床已确诊的肠癌病人及120例健康体检者血液标本，采用聚合酶链式反应(PCR)技术进行B19DNA检测。
Methods B19DNA in blood samples of 160 clinically confirmed large intestine cancer cases and 120 healthy persons were determined by PCR.
应用聚合酶链式反应（PCR）、多重PCR方法检测GSTM1和GSTT1在正常人群和结直肠肿瘤患者群体中的基因多态性分布。
The frequencies of GSTM1-null and GSTT1-null genotypes in colorectal tumor and control groups were ascertained by using polymerase chain reaction (PCR) and multiple-PCR techniques.
双轮回‘聚合酶链式反应’(PCR)和‘反向转录酶-聚合酶链式反应’(RT - PCR)被用来检测基因CYP1A1的转录水平。
A double - round reverse transcriptase - coupled polymerase chain reaction (RT - PCR) was applied to detect the CYP1A1 transcript.
方法采用聚合酶链式反应-单链构象多态性方法检测46例高血压患者和39例正常血压对照者的胰岛素受体基因第17、18外显子的多态性。
Methods The polymorphism if exon 17 and 18 of insulin gene were detected in 46 hypertension patients and 39 normal controls using PCR and single strand conformation polymorphism.
方法采用聚合酶链式反应-单链构象多态性方法检测46例高血压患者和39例正常血压对照者的胰岛素受体基因第17、18外显子的多态性。
Methods The polymorphism if exon 17 and 18 of insulin gene were detected in 46 hypertension patients and 39 normal controls using PCR and single strand conformation polymorphism.
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