目的：通过人输卵管上皮细胞共培养体系及EGF作用，探讨输卵管上皮及egf对受精卵体外发育的影响。
Objectives: to know the effect of human oviductal epithelial cells system and EGF on the vitro development of mouse embryos, according to the role of human oviductal epithelial cells system and EGF.
目的：建立体外研究精原干细胞与支持细胞共培养的细胞模型，探讨体外精原干细胞在支持细胞层上的增殖特点。
Objective: to construct cell model of spermatogonial stem cells (SSCs) with Sertoli cell layer in vitro, and study the proliferation characteristic of SSCs in vitro.
方法采用肿瘤抗原致敏的DC与CIK细胞共培养，分析其免疫表型，并用mtt染色法检测其对肿瘤细胞的杀伤力。
Methods Co-culture tumor antigen-sensitized dendritic cells (DCs) with cytokine induced killer (CIK) cells, analyze the immune epitope and determine the killing effect on tumor cells by MTT method.
以CZB为基础培养液，培养小鼠4、8 -细胞胚胎单卵裂球，研究葡萄糖、牛磺酸和猪输卵管上皮细胞共培养在其体外发育中的作用。
CZB medium was used as a base medium to study the function of taurine, glucose and porcine oviductal epithelial cells system in the culture of blastomeres isolated from mouse 4? 8-cell embryos.
结果表明，采用传统方法和共培养的方法并添加细胞因子均能分离获得类ES细胞。
The results indicated that ES like cells were both derived from the traditional method and co culture in medium with cell growth factors.
在含复合诱导成份的特殊共培养条件下，农杆菌能识别和大量附着到小麦悬浮细胞表面，并形成大量的纤维丝。
In a special medium containing complex-induced components, Agrobacteria were capable of recognizing and attaching to the surface of wheat cells, thus forming large amount of cellulose fibrils.
目的：建立稳定表达nt3的NIH3T3细胞株，通过体外共培养实验观察其对耳蜗螺旋神经节细胞生长的影响。
AIM: to establish a NIH3T3 cell line which expresses human NT3 and to observe the growth of cochlear ganglion cells by co culturing.
结合微流控技术，在同一基底上也可以达到多种细胞的共培养。
In combination with the microfluidic system, adhesion of multiple types of cells on a same substrate can be carried out.
目的：研究兔活性骨组织在共培养条件下对同种骨髓基质细胞来源成骨细胞黏附特性的影响。
AIM: To investigate the effects of rabbit living bone tissue by co-culture on adhesion characteristics of homogeneous osteoblasts from bone marrow stromal cell.
非共培养的2-细胞胚胎比率（86.4%）与琼脂包埋培养的2-细胞胚胎比率（88.7%）相比差异不显著；
The 2-cell embryo rate of direct culture group (86.4%) has no obvious difference in co-culture group (88.7%).
方法：体外法培养大鼠海马神经元细胞经不同剂量pcb共培养后，用MTT法观察海马神经元细胞生长、发育。
Methods: After rats' hippocampal cells were cultured with different PCB, MTT was used to observe the growth and development of hippocampal cells.
方法体外培养人胃癌MG C细胞，随机分为启膈方小、中、大剂量组及5-氟尿嘧啶(5-fu)组、空白对照组，共5组。
Methods MGC cell line of gastric cancer was cultured and divided into five groups randomly: low -, middle -, high-dose group of Qigefang, 5-fu group, control group.
应用细胞培养微量中和试验（固定病毒稀释血清法）对来自24个猪场共426份血清进行了猪伪狂犬病血清学调查。
The samples of 426 swine sera derived from 24 pig farms were detected for the neutralizing antibodies against swine pseudorabies Virus by micro neutralization test.
方法建立RPE与抗原特异性T淋巴细胞系和静止淋巴细胞之间的共培养系统。
Methods Co culture systems of RPE cells with antigen specific t lymphocyte lines and resting t lymphocytes were established in vitro.
在细胞培养的基底表面运用化学修饰，改变其对蛋白质和细胞的吸附特性，可以良好地控制不同细胞的共培养。
Using surface chemistry to change the substrate's ability to promote attachment of the proteins and cells, co-cultures of different types of cells can be achieved.
方法应用T细胞与皮肤组织体外共培养的方法检测银屑病T细胞对表皮通过时间产生的影响；
Methods Skin was cultivated in organ with T lymphocytes to investigate the effect of psoriatic T lymphocytes on epidermal turnover time.
结论嗅鞘细胞与大鼠尾状核神经元共培养可促进神经元生长。
Conclusions: OECs promote the growth of the co-cultured rat caudate nucleus neurons.
与人骨髓间充质干细胞培养上清共孵育。流式细胞仪检测表面抗原。
The monocytes were co-incubated with supernatant from human marrow(mesenchymal) stem cells culture, and flow cytometer was used to detect surface antigen.
盾叶薯蓣细胞与内生真菌re0105共培养第二代的性状与POD、PPO和PAL活性和过氧化物酶、多酚氧化酶、酯酶同工酶谱带与第一代相比比较稳定。
The second generation callus of co-culture of cell and RE0105 was stable in culture character, POD, PPO and PAL activities and isoenzyme bands of POD, PPO, EST.
方法大鼠胸主动脉平滑肌细胞传代培养，实验共分六组。
Methods Rat aortic smooth muscle cells were divided into 6 groups randomly.
目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
分别用小鼠、大鼠、猪的卵丘细胞与小鼠pno和NO进行了共培养。
Mouse PNO and NO were cultured with cumulus cells isolated from mouse, rat and swine, respectively.
最后，将纯化的生精细胞单独培养，同时还将纯化的生精细胞与支持细胞进行共培养。
Then, spermatogenic cells were purified. At last, the high purity spermatogenic cell was cultured single and with sertoli cell, respectively.
因此，树突状细胞(DC细胞)递呈肿瘤抗原给共培养的CIK细胞，希望可以增强CIK细胞识别肿瘤的能力以及提高其肿瘤杀伤活性。
Hence, dendritic cells (DC) were cultured and engineered to present tumor antigens to CIK cells hoping that this might enhance the recognition of tumor cells and its subsequent killing.
共培养的施万细胞和神经干细胞可以 3种方式接触生长 ：1 胞体与胞体接触 ；
Co cultured Schwann cells and neural stem cells have grown to touch together in following manner:1. Body touch body;
添加EGF，采用共培养方式成熟的卵母细胞，GSH含量高，超微结构更加健康。
There were high GSH concentration and healthy ultrastructure in EGF-added and coculture medium.
然而，当CEC培养上清经过浓缩和酸处理后，则在胸腺细胞共刺激实验中显示剂量-反应形式的抑制效应。
However, when the supernatant from CECs was concentrated and treated by acid activation, it exhibited the inhibitory effect of the thymocyte costimulation assays in dose-response way.
方法采用破骨细胞与骨片的体外共培养法和显微摄片、显微光密度仪扫描及计算机图像分析技术。
Methods Rabbit's osteoclastes were coincubated with bone slices and microphotographed, microdensitometric scanned and analyzed by computer image analysis system.
提示通过体外直接接触共培养，骨髓间充质干细胞能逆转髓核细胞退变，而且髓核细胞能诱导骨髓间充质干细胞向类髓核细胞转换。
Direct contact of MSCs and nucleus pulposus cell in vitro is essential for BMSCs reversing the nucleus pulposus cells degeneration and differentiating into nucleus pulposus-like cells.
提示通过体外直接接触共培养，骨髓间充质干细胞能逆转髓核细胞退变，而且髓核细胞能诱导骨髓间充质干细胞向类髓核细胞转换。
Direct contact of MSCs and nucleus pulposus cell in vitro is essential for BMSCs reversing the nucleus pulposus cells degeneration and differentiating into nucleus pulposus-like cells.
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