方法：采用细胞培养技术和组织工程学方法。
METHODS: Cell culture technique and tissue engineering method were used.
方法6个喉癌细胞系和4例喉癌旁正常组织上皮细胞培养。
METHODS Epithelial cell culture from 6 laryngeal carcinomas and 4 normal laryngeal tissues were conducted.
此外，讨论了用组织培养方法生产左旋多巴的可能性和固定化细胞培养等问题。
The possibility of the production of L-dopa by tissue culture of Mucuna and the problems in immobilized cell culture were discussed.
采用组织培养和单层细胞培养两种方法对SD大鼠的胰腺进行培养。
The tissue culture and the single layer cell culture techniques for SD rat pancreatic islets were studied.
结论：作者通过细胞培养和组织工程学的方法，建立了小型香猪角质形成细胞库。
CONCLUSION: The fibroblasts bank of mini fragrant pigs construct by using cell culture technique and tissue engineering method.
通过愈伤组织培养、悬浮细胞培养和原生质体培养方法对草坪草的一些种已建立较为完善的植株再生体系。
The plant regeneration systems of some turfgrass species have been established perfectly through callus culture, cell suspension culture, and protoplast suspension culture.
方法应用细胞培养、疫组织化学、荧光标记、光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
植物组织或细胞培养结合理化因素的处理，可大大提高变异的范围和频率。
The techniques, developed in 1970, for plant cell and tissue culture provide the selection of resistant varieties with an advanced method.
方法应用细胞培养、免疫组织化学、核荧光标记、荧光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
结果：倒置显微镜观察，悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。
Results: A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group.
采用比色法检测血清、淋巴细胞培养液和肌肉组织中的NO和NOS。
Colorimetry was used to detect the levels of NO and NOS in the serum, lymphocytic culture liquid and muscular tissue.
结果：不同类型的生物反应器及其机械性能、传质以及流体剪应力等对细胞培养组织的形态和功能产生的影响不同。
Results The bioreactors with different mechanical properties, mass transfer and fluid shear stress had different impacts on morphological and functional organizations.
应用PCR方法检测组织细胞培养物中的病毒增殖和增殖滴度，测定了PCV2病毒收获时间，试验表明PCV2病毒在PK15组织培养可连续传代，增殖病毒。
PCR method was applied to detect the proliferation condition and titer of PCV2 and to determine the harvest time of PCV2. The results showed that PCV2 could proliferate and passage on PK15 cells.
在植物体细胞组织培养中，蔗糖一直作为标准碳源，然而其他糖类物质如：葡萄糖、麦芽糖和山梨醇对植物体细胞培养也产生了一定的影响。
In somatic culture, sucrose has been a standard carbon source, however other kinds of sugar such as glucose, maltose and sorbitol have a certain influence on in vitro tissue culture.
通过愈伤组织培养、悬浮细胞培养、原生质体培养等手段，对早熟禾属、黑麦草属、结缕草属和剪股颖属的一些物种已建立了较为完善的植株再生体系。
Plant regeneration system of the species of genus Lolium, Poa, Zoysia, Agrostis and Festuca were established by means of solid medium culture, suspension culture, and protoplast suspension culture.
通过愈伤组织培养、悬浮细胞培养、原生质体培养等手段，对早熟禾属、黑麦草属、结缕草属和剪股颖属的一些物种已建立了较为完善的植株再生体系。
Plant regeneration system of the species of genus Lolium, Poa, Zoysia, Agrostis and Festuca were established by means of solid medium culture, suspension culture, and protoplast suspension culture.
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