采用冷胰酶消化法分离培养大鼠睾丸支持细胞，采用单细胞凝胶电泳法检测亚硝酸钠对大鼠睾丸支持细胞dna的损伤作用。
Method: The effects of sodium fluoride on DNA damage and repair in isolated male mice testicular cells were observed with single cell gel electrophoresis test (Comet assay).
目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
ACN对大鼠睾丸中ABP和抑制素基因表达水平有影响，提示丙烯腈对睾丸中支持细胞可能有损伤作用。
The results indicate that ACN could induce changes in the levels of ABP and inhibin gene expression in testes of male SD rats, that may damage Sertoli cells in testes.
最近研究表明，NO及NOS在雄性生殖系统分布广泛，NOS主要分布于睾丸间质细胞、支持细胞，少量分布于生精细胞。
The latest study showed that NO and NOS are widely distributed in male reproductive system. NOS is mainly distributed in Leydig′s cells and sertoli′s cells and a minor portion in spermatogenous cells.
最近研究表明，NO及NOS在雄性生殖系统分布广泛，NOS主要分布于睾丸间质细胞、支持细胞，少量分布于生精细胞。
The latest study showed that NO and NOS are widely distributed in male reproductive system. NOS is mainly distributed in Leydig′s cells and sertoli′s cells and a minor portion in spermatogenous cells.
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