本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
但什么东西可以使真核生物积聚如此之多、甚至有些多余的基因和蛋白质？
But what enabled eukaryotes to accumulate all these extra genes and proteins?
但是，所有这些DNA需要高能耗，大量成本甚至削弱巨大的细菌——阻止它们成为更为复杂的真核生物。
But all this DNA has a big energetic cost that cripples even giant bacteria — stopping them from turning into more complex eukaryotes.
像其他古生菌一样，这种古生菌的基因组同时与真核生物和细菌的基因组具有一些相似性。
Like other archaeans, their genomes share some similarities with both eukaryotes and bacteria.
最为熟知的应当是真核域，尽管对于地球生物圈而言，它可能并不是最重要的部分。
The most familiar domain, though arguably not the most important to the Earth's overall biosphere, is the eukaryotes.
染色体分离是真核生物正确传递遗传信息的关键。
The faithful chromosome segregation is crucial for transmitting the genetic information accurately in eukaryotic organisms.
纤毛虫是单细胞的原生动物，在进化上属于低等的真核生物，但其各细胞器之间囊泡运输系统极为完善。
Ciliate, a unicellular protozoa and low eukaryotes in evolution, has perfect vesicular trafficking system among organelles.
线粒体是有氧呼吸的场所，同时也是真核生物主要的能量产生中心。
Mitochondria are the sites of aerobic respiration, and generally are the major energy production center in eukaryotes.
为对真核基因的选择性剪接形式进行准确、快速、有效的研究，提出了一种启发式多序列比对算法。
A heuristic algorithm for multiple alignment was developed to more effectively study alternative splicing patterns of eukaryotic genes.
我们仍然需要解释的是更特化的真核生物的磷酸化途径是怎么进化而来并取代了细菌的系统。
What still needs to be explained is how the more typical eukaryotic phosphorylation cascades evolved and came to replace the bacterial systems.
古生菌还具有完全与细菌和真核生物不同的细胞膜。
Archaeans have cell membranes that are completely different from both bacteria and eukaryotes.
卷曲藻可能是一个菌落，或者一种真核生物——其包裹在细胞膜内的细胞功能已经出现分化的生物体。
Grypania may have been either a bacterial colony or a eukaryote - an organism with specialized cells, enclosed in a membrane.
科学家现在知道，这个共同的祖先，“第一真核生物”比任何已知的细菌都要复杂得多。
Scientists now know that this common ancestor, 'the first eukaryote', was a lot more sophisticated than any known bacterium.
科学家现在知道，这个共同的祖先，“第一真核生物”比任何已知的细菌都要复杂得多。
Scientists now know that this common ancestor , 'the first eukaryote', was a lot more sophisticated than any known bacterium.
在真核生物中， 泛素介导的蛋白降解途径参与了许多生物学过程。
Ubiquitin-mediated proteolysis is involved in many biological processes in eukaryotes.
结论野生型和突变型PRPF31基因真核表达载体的构建，为研究PRPF31基因突变引起视网膜色素变性的机制奠定了基础。
Conclusion Construction of the eukaryotic expression vectors of wild type and mutant PRPF31 genes is basic work for research on the mechanisms of retinitis pigmentosa caused by PRPF31 mutation.
遗传密码一度被视为所有生物皆同，今已发现在特定生物体中和某些真核生物的粒线体中有稍微不同的情况。
Once thought to be identical in all forms of life, the genetic code has been found to vary slightly in certain organisms and in the mitochondria of some eukaryotes.
真核生物对维持基因组的稳定性及防止非整倍体的产生，有其一套完整的保护机制，即细胞周期调控、检验点调控以及对损伤DNA的修复。
To ensure genome integrity and prevent aneuploidy, eukaryotes have evolved an integrated protective mechanism, including cell cycle control, checkpoint control, and DNA repair.
在真核生物中基因表达的调节主要是在转录水平。
Gene expression in eukaryotes is regulated primarily at the level of transcription.
作为其中的一个工作，真核生物基因启动子区域的识别是一个难点。
As a part of which, the recognition of promoter field in eukaryote's gene is very hard.
莱恩和马丁总结而这很可能解释为什么复杂的生命——真核生物——在地球的历史上仅进化了一次。
And that, Lane and Martin conclude, may well explain why complex life — eukaryotes — only evolved once in all of Earth's history.
目的构建水痘-带状疱疹病毒(VZV)糖蛋白e的真核表达载体。
Objective to construct the eukaryotic expression vector of varicella-zoster virus (VZV) glycoprotein e.
目的建立丙型肝炎病毒(HCV)复合多表位基因的真核表达载体，并在COS7细胞中瞬时表达。
Objective to construct an eukaryotic expression vector of compound multi-epitope gene of HCV and express the gene in COS7 cells.
真核生物中的许多蛋白质分子包含锌指结构区，这类蛋白称为锌指蛋白。
A number of proteins in eucaryon contain zinc finger domains and this class of proteins are called as zinc finger proteins.
Giardia和动物以及真菌几乎没什么历史渊源，有人认为它与最初的古真核生物很相似。
Giardia is very distantly related to animals and fungi, and some think it resembles the very first eukaryotes.
近年来，随着转基因动物制备技术的日趋成熟与完善，建立转基因动物模型已经成为研究真核基因表达调控的一种重要手段。
Recently, with the development of transgenic technology, producing transgenic animal model has become an important way to study the expression regulation of eukaryotic genes.
争论的结果不仅仅影响我们对早期真核生物进化的理解，还影响我们对贾第虫属生物学的观点。
The outcome of this debate affects not only our understanding of early eukaryotic evolution, but also our view of Giardia biology.
实验结果表明：该系统使用合适的真核表达载体提供脊灰病毒结构蛋白的技术路线是可行的。
The result indicated: in this system, the technique of eukaryotic expression vector providing poliovirus capsid protein was possible.
实验结果表明：该系统使用合适的真核表达载体提供脊灰病毒结构蛋白的技术路线是可行的。
The result indicated: in this system, the technique of eukaryotic expression vector providing poliovirus capsid protein was possible.
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