结果:成功地构建了重组BMP7逆转录病毒表达载体,并可在骨髓基质干细胞中表达BMP 7。
The viruses were used to infect directly BMSCs and the expression of BMP 7 gene in BMSCs was analyzed by immunohistochemical staining.
背景:基质细胞衍生因子是嗜淋巴细胞人类免疫缺陷病毒1感染的体外潜在抑制因子,可阻断人类免疫缺陷病毒1侵入人体的通路。
BACKGROUND: Stromal cell-derived factor 1 (SDF1), a potential inhibitor of infection by lymphophilic HIV-1 strains, can help to block the pathway of HIV-1 invasion into the human body.
重组病毒物理滴度测定后感染骨髓基质干细胞,在荧光显微镜下观察荧光强度。
After measuring virus titer, BMSCs were transfected and the fluorescence intensity was observed under fluorescence microscope.
同时亦可引起浅层基质发生细胞凋亡,以防止病毒从感染的上皮细胞传染至角膜深部。
After corneal infection, corneal epithelial cells can be induced to apoptosis, following that, the apoptosis is inhibited.
研究表明,在病毒感染过程中,狂犬病毒糖蛋白及基质蛋白均能诱导宿主细胞发生凋亡,有许多凋亡分子参与这个过程并起着作用。
It has been found that the ra - bies virus glucoprotein and matrix protein can induce cell apoptosis during the infection. Many cell apoptotic molecules are involved in the process.
将腺病毒感染的骨髓基质干细胞接种于修剪成一定形状的PLGA上,经电镜观察可见细胞贴附于材料表面和孔隙内并增殖。
Adenovirus-infected MSCs seeded onto PLGA showed high level of proliferation, and mass synthesis of cell matrix was observed with electronic microscoping.
将腺病毒感染的骨髓基质干细胞接种于修剪成一定形状的PLGA上,经电镜观察可见细胞贴附于材料表面和孔隙内并增殖。
Adenovirus-infected MSCs seeded onto PLGA showed high level of proliferation, and mass synthesis of cell matrix was observed with electronic microscoping.
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