三个候选甲基化差异通过靶向焦磷酸测序实验进行了验证。
Three candidate methylation differences were validated by targeted pyrosequencing assays.
目的：应用pcr -焦磷酸测序技术对食物中毒标本中沙门菌进行快速检测。
Objective: To apply PCR-Pyrosequencing technology on samples of food poisoning for rapid detection of Salmonella.
paabo博士说两年内他将得到尼安德特人的全基因组草图，同时他还设计了一种方法使得焦磷酸测序加快十倍。
Dr Paabo says he will have a rough draft of the entire Neanderthal genome within two years, and that he has devised a way to make pyrosequencing ten times faster.
由于受到进口仪器和试剂成本高昂的限制，焦磷酸测序技术的应用与仪器研发在国内才刚处于起步阶段。
Due to the high cost of importing the instruments and the relevant reagent, the applications of the techniques and the developments of the apparatus in the nation were just in the beginning stage.
本文以自主研发焦磷酸测序仪器为目的，对焦磷酸测序法的理论和焦磷酸测序仪在国内外的应用进行了广泛研究。
The purpose of the thesis is to develop a pyrosequencing device. The theory and the device's worldwide applications of the pyrosequencing were extensive researched.
本文的主要工作包括以下两个方面：1。 通过对比用于制备焦磷酸测序模板的几种方法，采用以不对称PCR作为制备测序模板的方法。
The main researches of the thesis are given as follows: 1. Several methods for preparing pyrosequencing template were compared, and the Asymmetry PCR method was employed to prepare the template.
对V6和V3可变区的焦磷酸测序，确定了3300- 5700个分类，这些分类总体上解释了超过99%的由这些样本获得的可变区的序列标签。
Pyrosequencing of the V6 and V3 variable regions identified 3,300-5,700 taxa that collectively accounted for over 99% of the variable region sequence tags that could be obtained from these samples.
采用单片机控制该系统运行，可以在PC端的人机界面设定试剂加样量和加样次数以及仪器运行的流程，为焦磷酸测序仪的开发搭建了一个初步的、具备一定基本功能的框架。
The platform was controlled by MCU and the GUI was developed to modify the parameters. The platform provides a basic and functional tool for the development of the device.
采用单片机控制该系统运行，可以在PC端的人机界面设定试剂加样量和加样次数以及仪器运行的流程，为焦磷酸测序仪的开发搭建了一个初步的、具备一定基本功能的框架。
The platform was controlled by MCU and the GUI was developed to modify the parameters. The platform provides a basic and functional tool for the development of the device.
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