最后我们选用异二丙胺锂（LDA）为碱，在干冰丙酮浴下，合成了烯醇磷酸酯，并有很高的立体选择性。
At last , we used the base: LDA, dry ice-acetone bath , to synthesize the enol phosphates and gain very high rigio-selectivity.
该试剂盒包含以下主要成分：磷酸烯醇丙酮酸羧化酶，丙酮酸脱氢酶和稳定剂。
The kit comprises the following main components: phosphoenol pyruvate carboxylase, pyruvate dehydrogenase and a stabilizer.
(PEPCK2或PEPCK - M)编码一种磷酸烯醇丙酮酸羧激酶(PEPCK)亚型，在肾和肝组织线粒体中发现该亚型(1)。
PCK2 (PEPCK2 or PEPCK-M) encodes an isoform of phosphoenolpyruvate carboxykinase (PEPCK) that is found in the mitochondria of renal and hepatic tissues (1).
PEPCK催化草酰乙酸成为磷酸烯醇丙酮酸的反应，这是代谢过程中的限速步骤(2)。
PEPCK catalyzes the reaction whereby oxaloacetate becomes phosphoenolpyruvate, a rate limiting step in the metabolic process (2).
其次，减缓代谢途径的一个中间体，通常是磷酸烯醇或柠檬酸降低酶的活性。
Secondly the enzyme is inhibited by an intermediate lower down the metabolic pathway, usually either phosphoenolpyruvate or citrate.
磷酸烯醇式丙酮酸(pep)是糖酵解中合成atp的两种磷酸基的供体之一。
Phosphoenolpyruvate (PEP) is one of the two phosphoryl group donors in the synthesis of ATP during glycolysis.
紫杉烯合酶是一种二萜环化酶，催化牛儿基牛儿基焦磷酸形成紫杉醇生物合成过程中的中间体紫杉烯。
Taxadiene synthase, a diterpene cyclase, catalyzes the conversion of geranylgeranyl diphosphate (GGPP) to taxadiene, a key intermediate in Taxol biosynthesis in yew.
磷酸烯醇丙酮酸羧化酶和依赖于NADP的苹果酸酶活性均低。
The activities of phosphoenolpyruvate carboxy-lase and NADP linked malic enzyme were found to be rather low.
本发明的试剂盒主要成分包 括：缓冲液、还原型辅酶、草酰乙酸、乙酰辅酶A、磷酸烯醇式丙酮酸羧化酶、丙酮酸脱氢酶及稳定剂；
The kit comprises the following main components: buffer solution, reduced coenzyme, oxaloacetic acid, acetyl coenzyme A, phosphoenolpyruvate carboxylase, pyruvate dehydrogenase and a stabilizer.
本发明的试剂盒主要成分包 括：缓冲液、还原型辅酶、草酰乙酸、酮戊二酸、磷酸烯醇式丙酮酸羧化酶、 异柠檬酸脱氢酶及稳定剂；
The kit comprises the following main components: buffer solution, reduced coenzyme, oxaloacetic acid, ketoglutaric acid, phosphoenolpyruvate carboxykinase, isocitric dehydrogenase and a stabilizer.
本发明的试剂盒主要成分包 括：缓冲液、还原型辅酶、草酰乙酸、酮戊二酸、磷酸烯醇式丙酮酸羧化酶、 异柠檬酸脱氢酶及稳定剂；
The kit comprises the following main components: buffer solution, reduced coenzyme, oxaloacetic acid, ketoglutaric acid, phosphoenolpyruvate carboxykinase, isocitric dehydrogenase and a stabilizer.
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