方法应用细胞培养、免疫组织化学、核荧光标记、荧光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
附着在合成超细纤维上的内皮细胞，微管、肌动蛋白和胞核已染色(2500倍)，荧光和激光共焦显微镜。
Endothelial cell attached to synthetic microfibers, stained with microtubules, F-actin and nuclei (2500x), Fluorescence, confocal.
能量色散x荧光分析仪作为一种重要的核分析工具，在地质、冶金、建材、环境等领域有着广泛的应用。
Energy dispersion X-ray fluorescence spectrometer (EDXRF) is an important nuclear analysis tool, which is widely used in the fields of geology, metallurgy, architectural material and environment.
利用荧光显微镜技术对紧露草雄蕊毛细胞的核穿壁现象进行了观察。
The technique of fluorescent ndcmp is used to observe the occurence of nuclear extrusion in Tradescantia stamen hairs.
能量色散X荧光分析技术是一种重要的核分析技术。
Energy Disperse X-ray Fluorescence Measurement (EDXFM) is an important nuclear analysis technique.
本文用荧光素双标记法研究大白鼠下丘脑向迷走神经背核簇和脊髓的投射。
The investigation was performed to demonstrate the projections of the hypothalamus to the dorsal vagal complex and spinal cord in the rat using the fluorescence double-labelling methods.
方法骨髓有核细胞免疫荧光标记后用流式细胞术分析。
Methods Bone marrow nuclear cells (BMNCs) were labeled by immunofluorescence and analyzed by flow cytometry.
方法用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化，并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白（GFAP）和增殖细胞核抗原（PCNA ）的表达水平。
Methods We measured the astrocyte cell cycles in different time after ischemia and anoxia by flow cytometry and detected the levels of GFAP and PCNA with fluorescence immunocytochemistry.
本研究还利用荧光标记和激光共聚焦显微镜相结合的方法，研究了HMC毒素对同核异质体玉米根冠细胞微丝骨架分布的影响。
The research was engaged in the effect of HMC toxin on microfilaments of N and C cytoplasmic male-sterile maize by fluorescence technique and confocal scanning microscopy.
荧光素钠法是核级高效空气过滤器现场试验的重要方法之一。
Sodium fluorescence method is an important method for on-site test of nuclear HEPA filters.
间接免疫荧光法检测血清中抗核抗体(ANA)的荧光滴度。
The serum fluorescence titres of anti-nuclear antibodies (ANA) were tested by indirect immunofluorescence technique.
为此，以X荧光分析仪为例，提出了一种实用的由太阳能光伏电池和锂电池联合供电的便携式核测量仪器电源设计方案。
Therefore, take X-ray fluorescence analyzer as example, a useful joint-power-supply consists of solar photovoltaic and lithium-ion cells is proposed.
免疫荧光实验结果指出，去除DNA和组蛋白的细胞核和染色体与抗肌动蛋白抗体呈阳性反应。
Indirect immunofluorescence tests showed that the DNA - and histone-depleted nuclei and chromosomes reacted positively with the anti-actin antibody.
本文应用HRP和荧光素标记法对大白鼠外侧隔核的传入性神经纤维联系进行了研究。
The afferent connections of lateral septal nucleus was studied in rat with HRP and fluorescence methods.
采用HRP、WGA-HRP及荧光双标记法，对68只大鼠隔区的主要亚细胞群隔内侧核、隔外侧核、斜角带核、隔三角核和隔微核之间的纤维联系和分支投射进行研究。
The interconnexions and branching projections between the various components of tne septalnuclear complex in 68 rats were studied with HRP, WGA-HRP and fluorescence double labeling methods.
免疫荧光染色后细胞纯度分析显示，A2B5染色阳性细胞占DAPI染核细胞的百分比为98.14%。
Purity analysis after immunofluorescence staining revealed that the ratio of number of A2B5-positive cells to that of DAPI-positive cells was 98.14%.
HPD荧光主要位于肿瘤细胞浆内，少量位于细胞核。
The fluorescence of HPD was observed densely in cytoplasm of tumor cells and faintly in nuclei.
核净未能引起荧光素酶活性值的增加，但菌核净能抑制DHT依赖的AR活性，并呈现。
In in vitro, DHT increased the AR activity and induced the expression of luciferase reporter gene in a concentration-dependent manner.
着丝粒和端粒DNA探针多色荧光原位杂交是一种较为精确的分析微核染色体组成的方法。
Multicolor FISH with centormeric and telomeric DNA probes was a precise technique for analyzing chromosomal composition of MN.
实验利用全细胞膜片钳技术研究了荧光染料核黄（NY）标记的支配牙髓的痛感觉神经元的电生理特征。
Whole-cell patch clamp technique was used to study the electrophysiological characteristics of nociceptive trigeminal ganglion (TG) neurons labeled by nuclear yellow (NY).
结果：三种孵育时间下，HMME荧光均呈胞浆弥散分布，且在核周附近的高尔基体灰度较高，细胞核几乎无荧光分布。
Results: For three incubation times, HMME fluorescence has diffuse cytoplasmic distribution, with little nuclear stain and brighter perinuclear area in Golgi apparatus.
方法：以Hep- 2细胞和猴肝切片为抗原基质，用IIF法检测10 5例SL E患者的抗核抗体，分析抗核抗体的荧光模式。
Methods Take HEP-2 cell and primate liver as antigen substrate, examine the ANA of 105 SLE patient's serum with IIF to analyse the immunofluorescent pattern.
目的通过对人退变髓核细胞的原代培养，了解其生物学特性，并将绿色荧光蛋白基因转染至细胞内。
Objective Human degenerative nucleus pulposus cells were cultured in monolayer, the biological action of the cells was observed.
共焦显微图象如荧光染色细胞核图象，往往是一种比较模糊的图象，并且亮度范围大，灰度均匀度也较差。
In most of cases, the laser scanning confocal microscopy images, such as fluorescence stained biomedical nuclei, are blurred and have a wide range of brightness and poor intensity homogeneity.
用荧光金逆行标记法观察大脑感觉运动区和脑干红核的神经元神经纤维再生；
The nerve fiber regeneration of neurons at sensorimotor area and rubrum nuclei in brain was observed with retrograde tracing of fluorogold;
斯氏按蚊雌蚊中肠壁外侧出现较强的亮绿色荧光， 肠壁细胞核仅出现样弱的亮绿色荧光。
Stronger fluorescent light appeared the outer of midgut wall, but a weaker in the nucleus of midgut wall cell.
该载 体是一种环状载体，包含原核复制起点、真核复制起点、筛选标记基因和绿色荧光蛋 白基因表达盒；
The vector is in ring shape , comprising a procaryon replication origin, two eucaryon replication origins and selective marker genes, and two green fluorescent protein gene expression cassettes;
真核表达的结果通过荧光染色的方法在流式细胞仪上进行检测，得到了明显的区分效果。
The consequence of eucaryon express proteinum binding with IBDV, then by fluorescence staining, detection on flow cytometer(FCM), we can gain a conspicuous discrimination result.
真核表达的结果通过荧光染色的方法在流式细胞仪上进行检测，得到了明显的区分效果。
The consequence of eucaryon express proteinum binding with IBDV, then by fluorescence staining, detection on flow cytometer(FCM), we can gain a conspicuous discrimination result.
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