其他细胞器标记酶活性在质膜组分中很低或没有。
Activitiesof marker enzyme for other organelles are low or not detectable in PMfraction.
综述了近年来常用于电化学酶联免疫传感器的标记酶及其在分析测试中的应用。
This paper deals in details with the recent development of labeled enzyme in EEIS, and the application in analytical Chemistry.
一系列的方法已被用来探讨这个问题，其中一个最常用的方法是研究女性肿瘤，她们是X连锁标记酶杂合子。
A variety of approaches have been used to address this issue, the most common of which is the study of tumors in women, who are heterozygous for X-linked marker enzymes.
酶免疫分析技术的质量依赖于抗原的纯度、抗体的特异性、合适标记酶的选用，其灵敏度取决于标记酶的高度纯化和高转化率。
The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label.
用脱氧核糖核酸末端转移酶介导的缺口末端标记技术（TUNEL）检测细胞凋亡。
Terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique were applied to deterimine the apoptotic cells.
大多数分子标记体系的利用都取决于聚合酶链反应（PCR），这是一项扩增特定DNA序列的重要技术。
Use of most molecular marker systems depends on the polymerase chain reaction (PCR), which is an important technique for amplifying specific DNA sequences.
由于很少有健康的细胞制造这些酶，所以它们是一种可靠的肿瘤标记物。
Few healthy cells make these enzymes, so they are a reliable marker of tumours.
该研究小组还利用新的探针开发了一种新的蛋白标记方法，称为探针的酶介导法(PRIME)。
The group also developed a new protein labeling method, called probe incorporation mediated by enzymes (PRIME), which utilizes the new probes.
肿瘤标记物通常是阴性。肝脏酶在正常和显著升高之间。
Tumor markers are generally negative. Liver enzymes range from normal to markedly elevated.
最近的一些研究表明环氧化酶2 (COX - 2)的表达可以作为发现乳腺癌细胞的标记。
Recent studies implicated expression of cyclooxygenase 2 (COX-2) as a marker to identify precursor cells for breast cancer.
限制性酶切片段长度多态性（RFLP）作为共显性的遗传标记，已广泛应用于遗传病的产前诊断。
As a codominant genetic marker, restriction fragment length polymorphisms, referred as RFLPs, has been widely Applied to the prenatal diagnosis of some genetic diseases.
方法：用APAAP免疫酶标法检测10例经il - 2激活的急性白血病患者骨髓细胞相关抗原标记及活化过程中的变化。
Methods: 10 patients with acute leukemia were selected and their bone marrow cell related antigen labeling and activation process changes activated by IL-2 were detected by APAAP method.
初步显示高效液相色谱蛋白酶解肽谱分析中，荧光检测硫代磷酸化荧光标记肽的可能性。
Therefore, the possibility to detect the thiophosphorylated and fluorophore labeled peptide during high performance liquid chromatography peptide mapping was primarily shown.
在一步层析检测中，不需要额外程序(如酶反应)来产生信号的标记是首选的。
Labels that do not require additional procedures (e. g., enzyme reactions) for signal generation are preferred in one-step chromatographic assay.
方法应用细胞培养、疫组织化学、荧光标记、光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
方法应用细胞培养、免疫组织化学、核荧光标记、荧光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
方法：用噻唑蓝（MTT）法、光镜及电镜、DNA凝胶电泳和原位DNA末端转移酶标记法，分别对耐药细胞株BIU-87／ADM及敏感细胞株BIU-87细胞进行了阿霉素诱导的细胞凋亡研究。
Methods: MTT assay, optical microscopy, electron microscopy, DNA agarose gel electrophoresis and in situ DNA nick end labeling were used to detect the apoptosis in BIU87/ADM and BIU87 cells.
结论：端粒酶可以作为口腔白斑癌变化学预防的替代性终点标记物监测口腔白斑癌变。
Conclusion: Ttelomerase could act as a surrogate endpoint biomarker for oral chemoprevention during OLK carcinogenesis.
由于形态学、细胞学、同工酶、RFLP等标记技术在大麦基因组中的多态性较低，限制了其应用。
Its applications were limited for the low polymorphism in the gene group of barley using the labeling technique of morphology, cytology, isoenzyme and RFLP.
本研究采用地高辛标记的RNA探针，用原位杂交的方法研究了大鼠胃底腺中胃蛋白酶产生细胞的个体发育。
In this study, the ontogeny of pepsinogen producing cells in rat fundic glands was studied by in situ hybridization using a digoxigenin-labeled RNA probe.
需要使用包括聚合酶链反应（PCR），琼脂糖凝胶电泳，探针的标记与检测等生物工程技术。
This technique employs standard operations of biomedical engineering, such as polymerase chain reaction(PCR), agarose gel electrophoresis, labeling and detecting of probe.
用脱氧核糖核酸末端转移酶介导的缺口末端标记技术（TUNEL）原位检测细胞凋亡。
Terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique were applied to deterimine the apoptotic cells in all the tissues mentioned above.
糖类分解代谢酶基因作为安全标记基因，近年来在植物转化中显示了巨大应用潜力。
The genes coding enzymes that catalyze special sugars have showed big practical potency in plant transformation as safe marker genes.
第二部分：利用金纳米粒子标记的DNA适配子为探针测定凝血酶的共振光散射分析。
Part two: Study on the Resonance Light Scattering analysis to determine thrombin by using gold nanoparticle-labeled DNA aptamer as probe.
观察各组家兔血清心肌酶学变化，并通过电镜形态学及原位末端标记法检测心肌细胞凋亡情况。
Then we observe the change of myocardium enzymatic dynamics and detect myocyte apoptosis with electronic microscope and situ end labeling way.
MMP - 2是肿瘤形成中的一种主要金属蛋白酶，可做为恶性表型的标记物。
MMP-2 is one of the main metalloproteinases active in neoplasia and is a marker of the malignant phenotype.
本试验以人工合成的榨菜和紫甘蓝离体嫁接嵌合体为试验材料，对其植株形态，酸性磷酸酯酶同工酶及分子标记等进行了研究。
In this study, the morphological characters of chimeras of tuber mustard and red cabbage were described, the acid phosphatase isozyme banding patterns and the molecular marker were analyzed.
成功的反义显像要求反义寡核苷酸易被细胞摄取、耐核酸酶、杂交稳定、标记简单。
The requirements for ideal antisense imaging include easy intakeing by cells, convenient labelling, stable hybridization and the tolerance against nuclease.
方法P CR -ELISA以地高辛标记端粒重复片段特异的探针与PCR变性产物杂交，通过ELISA检测端粒酶活性。
Methods We used telomerase PCR-ELISA an assay in which a digoxigenin labeled telomerase repeat-specific probe is hybridized to the PCR product detecting with ELISA.
方法P CR -ELISA以地高辛标记端粒重复片段特异的探针与PCR变性产物杂交，通过ELISA检测端粒酶活性。
Methods We used telomerase PCR-ELISA an assay in which a digoxigenin labeled telomerase repeat-specific probe is hybridized to the PCR product detecting with ELISA.
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