获得的阳性克隆进一步进行差异杂交筛选，去除假阳性。
The false positive clones were picked out by differential hybridization screen.
用吸附后的血清通过菌落原位免疫杂交筛选该基因组表达文库，从3000个菌落中获得了12个阳性克隆。
The genomic expression library was probed used the adsorbed-pooled sera, and 12 positive clones were obtained from 3000 postulating colonies by immunological hybridization.
种质资源是杂优猪选育的基础，育种部分是杂优猪选育的核心，完成专门化品系的选育、配合力测定和最优杂交模式的筛选以及专门化品系的纯繁扩群；
The genetic resource is the base of the breeding of hybrid swine, breeding section is the core, in which completes the breeding of special strains, combining ability testing .
方法采用减式杂交技术筛选大肠癌下调基因。
Method Subtractive hybridization technique was employed to screen down regulated genes of colorectal cancer.
可将4叶期和分蘖盛期的发根力作为选育或筛选杂交中稻强抗旱性品种的参考指标。
The rooting ability at 4 th leaf and at top tillering can be used as selection criterion in rice breeding for drought resistance.
目的应用抑制性消减杂交技术构建人肾癌差异表达基因文库并进行差异表达基因筛选。
Objective To screen the genes differentially expressed in human renal cell carcinoma (RCC) cell lines using suppression subtractive hybridization (SSH).
我们使用酵母双杂交的方法，筛选到BKI1的一个互作蛋白，即AT2G06010基因编码的蛋白。
In this paper, we use Yeast Two Hybrid System (Y2H) to screen the interacting protein of BKI1, and finally find AT2G06010.
红麻的器官培养和原生质体培养是品种快繁、变异体筛选以及体细胞杂交和遗传转化等生物技术育种的基础。
It's organ culture and protoplast culture is important basic technique of rapid clonal propagation, selection of imitative plants, somatic hybridization and genetic transformation.
为后续利用酵母双杂交技术筛选出ALP1的互作蛋白及构建互作图谱奠定了基础。
This will be used to screen for the interacting protein of ALP1 and construct the interaction map in the future.
本文着重探索转基因小麦中外源品质基因在杂交后代中的纯系筛选，同时分析外源基因在后代中的遗传规律。
This paper explores emphatically the selection of pure wheat lines. At the same time, the hereditary law of the foreign quality genes in the generations was also discussed.
结论：可利用远缘杂交优势，筛选和获得有效成分含量高的杂交新品种。
Conclusion: the advantage of far-origin hybridization can be used to select and obtain the new hybridization breed of high content of virtual components.
目前，原生质体培养在药用植物高产细胞系的筛选、体细胞杂交和基因工程方面已取得了长足的进展。
Recently, the protoplast culture of medicinal plants has mace great progress in the saltation of high productive cell lines, somatic cell hybridization and gene engineering.
是研究筛选抗ibd V单抗杂交瘤细胞的可靠方法。
This is a reliable method to select the hybridoma cells for McAb of IBDV.
这表明利用全糯小麦与黑粒小麦杂交能筛选出黑粒糯小麦新材料。
The results indicated that we could screen out new material of black kernel waxy wheat with the hybridization between whole waxy wheat and black kernel wheat.
从杂交水稻优异恢复系r 7954诱变筛选了1个富含抗性淀粉的突变体rs111。
The rice mutant enriched with resistant starch (RS), described as RS111, was induced from the elite hybrid rice restorer line R7954.
筛选共得到一株阳性杂交瘤细胞，命名为4d11。
用比较基因组杂交分析皮肤肿瘤高频率的染色体变异，将为相应肿瘤易感基因的筛选提供定位资料。
By analyzing altofrequent chromosome mutations, CGH can provide locating information for screening susceptibility genes of cutaneous tumors.
这个研究同时也包括了利用标准的CIMMYT铝敏感玉米系和相互杂交系进行铝毒筛选的对比评估。
These also included comparative evaluations involving al toxicity screening using a standardized CIMMYT al sensitive maize line and reciprocal hybrids.
基因是我所应用减数杂交技术筛选出的大肠癌新相关基因之一。
SNC19/ST14 gene was firstly cloned by subtractve hybridization method between normal mucosa cDNA and colorectal cancer tissue mRNA.
采用酵母双杂交系统，我们用LNX蛋白中的PD Z结构域为诱饵，筛选相互作用蛋白。
Using the PDZ domain in LNX protein as bait, we screened for LNX-interacting proteins by yeast-2-hybrid system.
从组合类型上看，大西洋与新型栽培种的杂交类型是最有希望筛选出适宜食品加工品种的组合类型。
For other crosses, further evaluations are needed. In view of cross type, Atlantic x Neo-tuberosum is more promising for development of processing potato cultivars.
前期试验采用芯片杂交技术从星星草中筛选出了一批在盐碱胁迫下高丰度表达的基因，其中包括代谢过程中的关键酶gapdh和OPDR。
Previously we have screened some highly expressed genes on saline-alkaline stress by microarray technology, including the key enzymes of metabolism GAPDH and OPDR.
结果表明，不同稻区稻瘟病菌菌株对籼、粳、杂交稻的致病力存在明显差异。筛选出一批毒力频率在20%以下，抗性较好的籼、粳稻新品种（系）和杂交稻新组合。
The pathogenicity of P. grisea isolates differ from various regions, and many cultivars (lines) or combinations are highly resistant to P. grisea and their virulent frequency are not more than 20%.
经同源重组后，以终点稀释和斑点杂交法筛选重组杆状病毒；
After co-transfection of recombinant plasmid and wild type AcNPV DNA, the rAcNPV was confirmed by the end point dilution assay and dot blot.
经同源重组后，以终点稀释和斑点杂交法筛选重组杆状病毒；
After co-transfection of recombinant plasmid and wild type AcNPV DNA, the rAcNPV was confirmed by the end point dilution assay and dot blot.
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