受精卵在含血清FBS或OCS培养液中的囊胚发育率明显高于在无血清培养基中的囊胚发育率。
Presumptive zygotes were cultured in mSOFaa medium supplemented with OCS or FBS had higher blastocyte yield than that in culture medium with free serum.
目的对无血清培养基与含血清培养基培养cho细胞进行比较。
Objective To evaluate the growth of CHO cells in serum-free and serum-containing media.
本发明提供了一种用于大规模培养CHO细胞的无血清培养基。
The present invention provides a serum free medium used for cultivating CHO cellular extensively, and discloses the prescription of the culture medium.
在培养后期换用无血清培养基培养，细胞生长及蛋白表达水平正常。
During the later period of the cultivating process, the applying of serum free medium had no significant effects on the cell growth and protein expressing.
结论目前无血清培养基尚不能完全取代含血清培养基用于培养CHO细胞。
Conclusion At present, the serum-containing medium for culture of CHO cells can not be completely replaced by serum-free serum.
方法从人胚胎海马区分离神经干细胞，采用无血清培养基，进行体外扩增培养、传代。
Methods The serum free culturing technology was used to isolate, culture and pass neural stem cells from embryonic human hippocampus.
方法：应用无血清培养基采用小方瓶培养CHO细胞，观察细胞维持时间、培养过程的形态变化。
Methods:Culture CHO cells in serum-free media and then compare the maintainance time, morphological changes and hemagglutination titers of the cells in culture supernatant.
我们提出了一种新颖的三阶段方法有效地指示的人胚胎干细胞的分化成肝细胞在无血清培养基中。
We report a novel three-stage method to efficiently direct the differentiation of human embryonic stem cells into hepatic cells in serum-free medium.
该培养基与商品化的无血清培养基比较，能够使细胞生长维持较长的时间，表达产物分泌量也相对较高。
In comparison with commercial SFM, it can maintain growth of CHO cell for much longer peroid and the expressing level of HBsAg is higher too.
通用的昆虫细胞培养基配方的不断优化，特定昆虫细胞株培养基的研制和适用于大规模悬浮培养的无血清培养基的商品化；
Instect cell culture media were optimized carefully to find new medium for special cell strain and serum free media was manufactured commercially for suspension cell mass production;
含有人MMP - 9的无血清培养基经过部分纯化，浓缩后使用MMP - 9抗体进行western blot分析。
Western blot analysis of partially purified, concentrated, serum-free medium containing human MMP-9, using MMP-9 Antibody.
在这个无血清培养基中，SSV -NIH3T3细胞可以很好地生长，并具有分泌血小板生长因子样生物学活性物质和致裸鼠肿瘤的能力。
In this SFM SSV-NIH3T3 cells grow well, they keep the ability of secreting platelet-derived growth factor-like material into culture medium and causing tumor growth in nude mice.
结论无蛋白培养基可完全取代常规的有血清培养基，用于生产高滴度、高纯度单克隆抗体。
Conclusions CD medium is able to replace serum containing medium and very suitable for production of high quality McAb.
采用无血清液体培养基、DMEM培养基和MEM培养基进行PK15细胞的培养，观察细胞增殖及传代效果，确定无血清液体培养基的营养功能。
PK15 cells were cultured with free-blood serum media, DMEM media and MEM media in other to observe the effect of cell proliferation, cell transfer and define the ability of nutrition.
采用无血清液体培养基、DMEM培养基和MEM培养基进行PK15细胞的培养，观察细胞增殖及传代效果，确定无血清液体培养基的营养功能。
PK15 cells were cultured with free-blood serum media, DMEM media and MEM media in other to observe the effect of cell proliferation, cell transfer and define the ability of nutrition.
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