除了具有标记为无菌通道的设备之外，所有的设备均须使用培养基直接接种法进行检测。
All devices, with the exception of devices with Pathways Labeled Sterile, are tested using the Direct Inoculation of the Culture Medium method.
如果膜过滤技术是不适合的，则使用在供试产品无菌检查法项下的培养基直接接种法。
If the membrane filtration technique is unsuitable, use the Direct Inoculation of the Culture Medium method under Test for Sterility of the Product to be Examined.
只要其性质许可，这些药品将使用供试产品无菌检查法项下的膜过滤法来检测。
Pharmacopeial articles are to be tested by the Membrane Filtration method under test for Sterility of the product to be Examined where the nature of the product permits.
目的探讨薄膜过滤法在小容量注射剂无菌检查中应用的可行性。
Objective:To determine the validity of membrane filtration method in the sterility test of little capacity injection.
结果：确定了注射用阿莫西林钠克拉维酸钾无菌检查法的最佳冲洗条件及操作方法。
Result: the best washing condition and operation method for the sterile test of Amoxicilin sodium and Clavulanate potassium for injection were determined.
用此无菌培养试验法进行的水稻有机氮营养试验证明，水稻能直接吸收根际营养液中的氨基酸。
Results from sterile experiment for rice organic N nutrition showed that amino acid could be directly taken up by plants.
建立复方磺胺嘧啶锌乳膏无菌检查法。
A sterility test method for the Compound Sulfadiazine Zinc Cream was established.
结果：确定了亚胺培南一水合物无菌检查法的最佳冲洗条件及操作方法。
Results: the best washing condition and operation method for the sterility tests of Imipenem Monohydrate were determined.
方法：无菌检查法的验证试验——抑细菌和抑真菌试验及冲洗量试验。
Methods: The validation for sterility test includes inhibition test for bacteria and fungi and irrigation test.
方法按《中国药典》2005年版附录无菌检查法项下薄膜过滤法。
Methods the method was based on the membrane filtration of sterility test of Chinese Pharmacopoeia 2005.
结论：建立的亚胺培南一水合物无菌检查法可供实用。
Conclusion: The established sterility test method for Imipenem monohydrate may be practical.
结论采用该法对烧伤一号油剂进行无菌检查，方法可行，结果准确。
Conclusion the established sterility test method of the Burn I oil agent is scientific and accurate.
结论：该法能消除注射用头孢美唑的抑菌作用，使无菌检查结果准确、可靠。
Conclusion:The method mentioned in this study can eliminate the antibacterial effect of injecting cefmetazole, therefore the examination was of nicety and reliability.
无菌条件下取正常足月剖腹产的脐带近胎儿段，剔除动静脉，取出其中的间质组织，胶原酶消化法原代培养细胞进行培养和纯化获得贴壁细胞。
Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.
本试验参照《中国药典》2005年版二部无菌检查法进行试验。
The test method was carried out according to the method in volume two, Chinese Pharmacopoeia Edition 2005.
无菌取小鼠脾脏，不锈钢网制备脾细胞溶液，尼龙棉柱法分离T淋巴细胞；
The splenical cells of mice were prepared through a stainless steel mesh and T lymphocytes were purified through a nylon wool column.
方法采用直接接种法，通过无菌验证试验确定无菌检查方法的有效性。
Conclusion The sterility test reassured by verification test is feasible and reliable.
结果与结论细胞因子类制品不同的品种和剂型可以采用不同的无菌检查法，并对新建立的无菌检查法进行方法学验证。
Results and Conclusion Different varieties and formulations of cytokines could apply dissimilar methods in sterile test. Methodology validations were performed for these new established tests.
结论：经方法学验证，醒脑静注射液无菌检查法准确可靠。
Conclusion: The method is accurate and reliable. It can be used for sterility test of Xingnaojing injection.
本试验取硫酸庆大霉素注射液，按《中国药典》2005年版二部附录所载的“无菌检查法”项下进行方法学验证试验。
According to the "sterile test method" which is recorded in the appendix in Chinese Pharmacopoeia (2005 Edition), the methodology validation is experimented with Gentamycin sulfate injection.
它还配以适量稀释剂、助剂，采用喷雾干燥法、冷冻干燥法及直接混合法制备得到注射用无菌粉末。所述的方法不同其处方也不同。
Besides, said sterile powder for injection also can contains proper quantity of diluent and adjuvant, and is made up by adopting spray-drying process, freeze-drying process and direct mixing process.
以玛咖无菌苗叶片为材料，通过改良的异硫氰酸胍法提取其总rna。
By using the improved isothiocyanate method, total RNAs were extracted from leaves of sterile Maca plantlet.
方法 ：将摩西球囊霉接种于半夏无菌组培苗，于不同时间取样，通过染色镜检法观察丛枝菌根形成过程。
Method : The tissue culture plantlets of P. ternata were inoculated with G. mosseae, the formation of AM were sampled and observed with microscopy by staining.
方法 ：将摩西球囊霉接种于半夏无菌组培苗，于不同时间取样，通过染色镜检法观察丛枝菌根形成过程。
Method : The tissue culture plantlets of P. ternata were inoculated with G. mosseae, the formation of AM were sampled and observed with microscopy by staining.
应用推荐