该方法涉及从血浆中固相提取胰岛素，以及用荧光胺对胰岛素进行柱前衍生。
The method involved solid-phase extraction of insulin from plasma and pre-column derivatization of insulin with fluorescamine.
方法：溶剂提取及各种柱色谱法分离，光谱技术鉴定结构。
Method: The compounds were extracted with solvents, isolated by various column chromatography and identified by spectroscopic methods.
采用溶剂提取和柱色谱法分离纯化市售大豆粉末磷脂（卵磷脂含量14.05%），得到高纯度的卵磷脂产品（纯度92.80%）。
Phosphatidylcholine of high purity(PC, content 92.80%) was prepared from market soybean power phospholipids(PC content 14.05%) by using solvent extraction and column chromatography.
作者主要探索用离子交换树脂柱析法提纯文蛤提取液中牛磺酸及用吸收光谱法测定牛磺酸的可行性。
This paper is to discuss the feasibility of refining taurine in the extract of Meretrix meretrix by column chromatography and determination of taurine by spectrophotometry.
方法首先用溶剂萃取结合大孔吸附树脂分离，然后用硅胶柱精制提取。
Methods Silica gel superfine process was conducted by combining solvent extraction and separation with macroporous absorbent resin.
有机氯类用乙酸乙酯—环己烷和凝胶净化柱提取净化，石英毛细管气相色谱(配ecd检测器)检测。
Organic chlorine using ethyl acetate - cyclohexane and gel purification column extraction purification, quartz capillary gas chromatography (ECD allocation) detection.
方法：比较样品提取中不过聚酰胺柱以及过不同粒度的聚酰胺柱的柚皮苷的含量，以确定最合适的方法。
Method:To establish the most appropriate method by comparing the content of Narrigin in sample which did not pass polyamide column and passed various granule grades polyamide columns.
猪肝样品匀浆后用稀盐酸提取，经免疫亲和色谱柱纯化，再用GC/MS检测，即为IAC-GC/MS法。
After extraction with dilute HCl, the extracts of swine liver homogenization was loaded on the IAC column for purification and determined by GC/MS.
纯化阶段，硅胶柱色谱及制备型薄层色谱处理提取物，正己烷沉淀结晶。
In purifying phase, the extracts were disposed by silica gel column chromatography and preparation lamella chromatography, then crystaled by N-Hexane.
方法：（1）以硅胶柱色谱法，乙酸乙酯-石油醚梯度洗脱，自何首乌中提取分离二苯乙烯苷单体成分。
Methods: (1)To separate stilbene glycoside by using silica gel column chromatography with gradient elution consisting of ethyl acetate and petroleum ether.
方法：溶剂提取和硅胶柱色谱法进行分离，理化性质和光谱数据鉴定其结构。
Method: Compounds were separated by solvent extraction and column chromatography with silica gel and structures were elucidated by chemical evidence and spectral analysis.
同时，介绍了分析所用的色谱柱、流动相、使用的检测手段以及样品的提取方法；
The chromatographic columns, mobile phases as well as detecting devices and sampling devices used in HPLC for this purpose were described.
有机磷经丙酮提取，凝胶层析柱净化，HP - 5石英毛细管色谱柱分离，火焰光检测器检测。
Organic phosphorus by acetone extraction, gel column chromatography purification, HP-5 silica capillary column chromatography, flame-ray detector.
该方法经优化后的条件为：样品经乙腈超声提取，弗罗里硅土柱净化，DAD二极管阵列检测器，经高效液相色谱测定。
The optimized conditions were that the sample was extracted by ultrasonic acetonitrile, cleaned up by Florisil-phase extraction (SPE). HPLC with DAD detector was used for determination.
方法采用乙醇提取，石油醚脱脂，氯仿萃取，上聚酰胺柱得到高良姜素。
Methods Galangin was extracted with ethanol, degreased with ligarine, and extracted with chloroform.
方法青蒿经石油醚提取浓缩，浸膏经过硅胶柱，用不同配比的乙酸乙酯洗脱，用tLC法跟踪，UV法检测。
Methods Artemisinin is extracted with petroleum ether and pass column of silica gel and gradient elution with ethyl acetate, then it is tracked with TLC and detected with UV-VIS.
方法：应用本层析柱方法从金鱼组织所提取RNA和采用异硫氰酸胍-酚-氯仿一步法提取RNA的比较。
Methods: To compare the method of the extract of RNA from goldfish using laminar analysis column with that of using one-step guanidinium isothiocyanate-alcohol phenyl-chloroform.
在浅层土壤中，EDTA对酸可提取态金属的萃取尤为显著，但随着土壤柱深度的增加，酸可提取态最容易被重新吸附。
In shallow soil, EDTA acid extractable metals extraction is particularly significant, but with the increase of soil column depth, acid extractable most easily re-adsorption.
方法：硅胶柱色谱法，乙酸乙酯-石油醚梯度洗脱，自何首乌中提取分离二苯乙烯苷单体成分。
Methods: (1) to separate stilbene glycoside by using silica gel column chromatography with gradient elution consisting of ethyl acetate and petroleum ether.
方法对新疆雪莲全草乙醇提取物的各个萃取部位进行反复柱色谱，根据光谱数据和理化性质确定化合物结构。
METHODS The constituents of EtOH extraction from the whole plant of Saussurea involucrata were isolated and purified by repeated column chromatography.
方法：用纯净水提取，溶剂萃取，大孔吸附树脂和硅胶柱色谱分离等方法分离并纯化亚麻根的微量成分，通过波谱分析鉴定化合物的结构。
Method: Isolation and purification of the trace constituents were carried out mainly by solvents extraction and macroporous adsorbing resin and silica gel column chromatography.
通过采用综合分析，将可疑成分提取、分离、 柱色谱纯化，结合NMR、 ICP_AES和元素分析，确证了汤样中的致毒物质是沙蚕毒素类农药“ 杀虫环”。
The components were comprehensively assayed by 1H NMR, ICP _ AES and elemental analysis. They proved to be a pesticide of thiocyclam and its by _ product.
通过采用综合分析，将可疑成分提取、分离、 柱色谱纯化，结合NMR、 ICP_AES和元素分析，确证了汤样中的致毒物质是沙蚕毒素类农药“ 杀虫环”。
The components were comprehensively assayed by 1H NMR, ICP _ AES and elemental analysis. They proved to be a pesticide of thiocyclam and its by _ product.
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