该文对嗜硫色谱及其在抗体纯化中的应用做了综述。
The review introduces the method for the purification of antibodies and covers the development and application of the original technology.
亲和色谱是用于抗体纯化的有效方法，本文对亲和色谱配体的研究进展进行了综述。
Affinity chromatography is a powerful tool used in the purification of antibodies. In this paper, different kinds of affinity ligands used in affinity chromatography are described.
去糖基化后，小麦PGTP与天然纯化的 PGTP产生的抗体不发生相互作用。
After deglycosylated, wheat PGTP could not interact with antibody produced by purified native PGtP from wheat.
抗体由蛋白A和肽段亲和层析纯化得到。
Antibodies are purified by protein A and peptide affinity chramotography.
多克隆抗体由合成化肽段免疫动物，该合成肽段与YAP人源蛋白序列一致，抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of YAP. Antibodies are purified by protein a and peptide affinity chromatography.
多克隆抗体通过用DYKDDDDK合成肽免疫动物制备。抗体用蛋白a和多肽亲和色谱法纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein a and peptide affinity chromatography.
纯化的抗体不能直接用于其它已知抗原的检测，其功能是作为兔源抗体的同型对照。
The purified antibody is not directed against any known antigen. It functions as an isotype control for rabbit antibodies.
数天后，技术人员剪下树叶，磨碎，纯化抗体。
After a few days, technicians snip off the plant's leaves, grind them up and purify the antibody.
去糖基化的小麦PGIP不能与纯化的天然PGIP产生的抗体发生免疫学反应，可能是由于小麦PGIP中寡糖链为抗原决定部位。
After deglycosylated, wheat PGTP could not interact with antibody produced by purified native PGtP from wheat. It is the glycans of wheat PGIP that probably fonn the epitope.
抗体由蛋白a和肽段亲和层析纯化得到。
Antibodies are purified by protein a and peptide affinity chromatography.
结论用尼龙滤膜zbm改进的膜亲和层析法，可用于纯化腹水中的单克隆抗体，且简便、省时、有效。
Conclusion The modified MAC with ZBM is a much easier, time-saving and effective method for affinity purification of antibodies in ascites.
探索具有可放大的、非特异性纯化目的抗体片段的工艺路线。
To explore the antibody fragment purification technics that is befitting for production scale.
目的制备和纯化抗人大肠癌单克隆抗体(ND - 1)，分析其在大肠癌诊断中的应用价值。
Objective To prepare and purify a new monoclonal antibody (ND-1) against human colorectal cancer and analyze its application in diagnosis of colorectal cancer.
目的：制备并纯化抗核糖核酸酶抑制因子(RI)的抗体。
AIM: to prepare and purify the antibody against ribonuclease inhibitor (ri).
方法：在表达及分离纯化VEGF的基础上，成功地制备了VEGF抗体，以夹心法测定vegf。
Methods: Based on isolation, purification and expression of VEGF, successfully prepared anti VEGF antibody and developed a sandwich radioimmunoassay for VEGF.
目的用单克隆抗体亲和色谱从人血小板破碎液中纯化血小板第4因子(PF4)。
PurposeThe aim is to purify human platelet factor 4 (PF4) using a monoclonal antibody affinity chromatography.
纯化的抗体不能直接用于其它已知抗原的检测，它是从天然兔血浆中分离出来的，通过蛋白A亲和色谱法制备。
The purified antibody is not directed against any known antigen. It was isolated from naive rabbit sera and prepared by Protein A chromatography.
⒉ 了解多克隆抗体的制备及纯化的基本方法。
Master the methods of preparation and purification of polyclonal antibody.
其中，已对从牛脑热休克蛋白纯化提出的多克隆抗体，检测人类热休克蛋白质。
Polyclonal antibodies which had been raised against HSPs purified from bovine brains were used to detect human HSPs.
酶免疫分析技术的质量依赖于抗原的纯度、抗体的特异性、合适标记酶的选用，其灵敏度取决于标记酶的高度纯化和高转化率。
The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label.
抗体采用蛋白a和肽亲和层析法纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
用亲和层析纯化的猪繁殖与呼吸综合征病毒(PRRSV)重组核衣壳蛋白gst - N作为诊断抗原，建立检测prrs血清抗体的间接ELISA方法。
Using recombinant nucleocapsid protein GST-N of porcine reproductive and respiratory syndrome virus (PRRSV) as antigen, an indirect ELISA for the detection of antibodies against PRRSV was developed.
抗体由蛋白a和肽段亲和层析技术纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
该多克隆抗体由合成的人类CDK5氨基酸序列对应肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human CDK5. Antibodies are purified by protein A and peptide affinity chromatography.
该抗体通过使用合成肽段免疫动物而获得，并经蛋白a和肽亲和层析纯化。
Antibodies are produced by immunizing animals with synthetic peptides, and are purified by combinations of Protein a and peptide affinity chromatography.
该多克隆抗体用与人类PKM2蛋白氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PKM2. Antibodies are purified by protein A and peptide affinity chromatography.
该多克隆抗体用与人类LDHA氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白亲和层析纯化而得。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human LDHA. Antibodies are purified by peptide affinity chromatography.
建立了间接荧光免疫法检测纯化抗体的方法。
An indirect fluorescence immunoassay to detect antibody was developed.
多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与包围于人源APC蛋白序列一致，抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human APC. Antibodies are purified by protein a and peptide affinity chromatography.
亲和纯化后抗体的纯度达99%以上。
After purification, the purity of the moAbs could reach more than 99%.
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