应用免疫组化方法检测增殖细胞核抗原和P-选择素的表达。
The local expression of proliferating cell nuclear antigen(PCNA) and P-selectin were analyzed by immunohistochemical technique.
方法：应用免疫组化方法检测73例晚期喉癌中PCNA表达、病理学方法检查颈部阳性淋巴结数。
Methods:Expression of PCNA and the number of positive nodes were determined in 73 cases of local advanced laryngeal cancer with immunohistochemical and pathological technique.
应用免疫组化方法检测各组织中COX - 2、VEGF的表达水平及其微血管密度(MVD)。
The expression of COX-2, VEGF and the microvessel density (MVD) were detected by means of immunohistochemistry.
方法应用免疫组化方法观察大鼠局灶性脑缺血后脑组织中FAP - 1蛋白免疫反应阳性细胞数量及分布。
Methods the distribution and quantity of FAP-1 immunological positive cells in the brain were observed with immunohistochemistry method after focal ischemia.
应用免疫组化方法检测治疗后各组肿瘤组织中环氧合酶-2（COX-2）和血管内皮生长因子（VEGF）的蛋白表达；
Then the volume and weight of tumor, the post-therapeutic weight of mice were studied after 28 consecutive days. The COX-2 and VEGF expression of tumors were detected by immunohistochemistry.
方法：应用免疫组化方法检测72例喉鳞状细胞癌组织标本中MMP-2和MMP-9蛋白的表达，并与临床病理特征和预后进行对比。
Methods: Immunohistochemistry was used to examine the expression of MMP-2 and MMP-9 in tissue samples of 72 LSCCs and the results were compared to clinical pathological features and prognosis.
方法：应用免疫组化法检测乳腺癌及相应癌旁组织tlr4、增殖细胞核抗原(pcna)的表达，TUNEL法检测组织中细胞的凋亡。
Methods: the expression of TLR4 and PCNA in cancer tissues and adjacent tissues were assessed by immunohistochemical method and the apoptosis was detected by the TUNEL method.
应用粘液组化及免疫组化方法对104例胃癌的异质性进行了研究。
The heterogeneity in 104 cases of gastric carcinoma were studied by immunohistochemical and mucin histochemical techniques.
方法应用免疫组化PABC法，分别对19例翼状胬肉标本和5例正常结膜组织中的MUC5AC表达进行检测。
Methods The immunohistochemical methods PABC was performed in study of expression of MUC5AC in 19 pterygial specimens and 5 normal conjunctiva.
方法应用免疫组化技术检测慢性心肌炎和扩张型心肌病（DCM）心肌组织中CAR的表达；
Methods The immunohistochemistry was applied in examining the expression of CAR in myocardium of dilated cardiomyopathy(DCM) and chronic myocarditis.
方法应用免疫组化SABC法检测45例人胃癌组织中COX - 2、VEGF的表达，并以30例正常胃黏膜组织作为对照。
Methods The expression of COX-2 and VEGF were studied by immunohistochemistry SABC method in 45 gastric carcinoma specimen and 30 normal gastric tissues as control.
方法：应用免疫组化和形态计量方法，检测80例骨肉瘤TAF表达和肿瘤微血管密度。
Methods The expression of TAF and microvascular density count in 80 osteosarcomas were investigated by immunohistochemical staining and morphometric technique.
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
应用SP免疫组化方法观察63例大肠癌细胞外基质成分层粘连蛋白（LN）、IV型胶原的表达。
The expression of two components of extracellular matrix, Laminin(LN)and type IV collagen(IV c)in 63 cases of colorectal carcinomas were studied using SP immunohistochemical method.
以免疫组化方法检测近端胃黏膜5 - HT阳性细胞，应用图像分析系统测定其积分光密度(IOD)值。
Proximal gastric mucosal 5-ht-positive cells were examined by immunohistochemical method and their integrated optical density (IOD) value measured by image analysis.
方法应用SP微波免疫组化技术和统计学分析。
Methods SP microwave immunohistochemical technique and statistical analysis were practiced.
方法：建立C6/SD大鼠胶质瘤模型后，应用流式细胞仪检测、免疫组化、HE染色三种方法，研究C6胶质瘤细胞的增殖动力学指标。
Methods: After establishing a C6/SD glioma model, by using flow cytometric analysis, immunohistochemistry and HE stain three methods, the proliferation kinetics in C6 glioma cell was measured.
方法：应用免疫组化SABC法进行检测。
方法：应用HE染色，组化及免疫组化标记对9例PCMT进行了形态学观察，并对其临床资料作了分析。
Methods Pathomorphology of 9 cases of PCMT was observed with HE staining?histochemistry and immunohistochemistry, and their clinical data were analysed.
方法应用免疫组化法对101例大肠癌组织石蜡标本进行MAGE-3抗原表达测定。
Method Immunohistochemical technique for MAGE-3 antigen was performed in paraffin embedded sections of 101 CRC specimens.
方法应用免疫组化SP法检测61例胃癌组织及其癌旁组织fak的表达。
Methods Immunohistochemical method by SP was used to detect FAK expression in the cancer tissue and the resection margin of 61 patients.
方法：应用SP免疫组化法，检测P 21, P27等细胞周期依赖性激酶抑制剂和增殖细胞核抗原(pcna)在角膜上皮不同部位的表达情况。
METHODS: the expressions of CKI, P27, P21 and proliferating cell nuclear antigen (PCNA) were tested in different regions of corneal epithelium by SP immunohistochemistry.
方法应用免疫组化和形态计量方法，检测80例骨肉瘤VEGF表达、肿瘤微血管密度（MVD），以及OS-732血管生成相关因子的表达。
Methods The expression of VEGF and intratumoral microvascular density(MVD) in 80 osteosarcomas and osteosarcoma cell OS-732 was detected by immunohistochemical staining.
方法：应用免疫组化的方法(SP法)观察了HLA -DR抗原在15例斑秃皮损中及8例正常头皮组织中的表达情况。
Method: Immunohistochemical technology (sp method) was used to detect the HLA-DR antigens in scalp specimens from15 cases of AA and 8 normal controls.
方法应用免疫组化和RT-PCR法，以正常皮肤、正常疤痕为对照，检测增殖性疤痕中vegf表达，并研究VEGF与表皮细胞增殖、血管新生的相关关系。
Methods VEGF protein secretion and VEGFmRNA expression were detected by immunohistochemistry and RT - PCR, and were compared with cell proliferation and revascularization.
方法：应用免疫组化染色s - P法，检测88例乳腺癌组织石蜡切片中MMP9及CD 147的表达情况。
Methods: Expression of MMP9 and CD147 protein in 88 patients with breast cancer was measured by immunohistochemistry (S-P) method.
方法：应用免疫组化染色s - P法，检测88例乳腺癌组织石蜡切片中MMP9及CD 147的表达情况。
Methods: Expression of MMP9 and CD147 protein in 88 patients with breast cancer was measured by immunohistochemistry (S-P) method.
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