• 早期阶段的幼胚培养成苗与外源激素有一定关系。

    Plants normally grown at early stage in young embryos are related to exogenous hormones.

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  • 在原胚及幼胚胚原细胞质中也呈现很弱的正反应。

    The embryo initials and suspensor cells showed very weak Feulgen positive reaction in the proembryo and young embryo.

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  • 结果表明:幼胚在MT培养基上直接成苗质量最好;

    The results showed that seedlings directly generated from young embryos were the strongest on the solid MT medium.

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  • 研究了温度与棉花生长发育及幼胚离体培养的关系。

    The relationship between air temperature and plant grow th and development and embryo development of cotton was studied.

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  • 基因型差异是影响小麦幼胚一步成苗培养的主要因素。

    The difference from the gene type of wheat is an importance factor effecting on the one-step culture for immature embryos of wheat.

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  • 以杂交鹅掌楸幼胚为外植体,进行体细胞胚胎发生研究。

    Somatic embryogenesis was successfully done in Liriodendron hybrids with the immature zygotic embryos as explants.

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  • 观察了大麦雌雄性配子的融合过程及其幼胚的早期发育。

    The process of fertilization and early embryogenesis in barley was observed.

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  • 利用基因枪法,以菜豆几丁质酶基因转化小麦幼胚愈伤组织。

    Using embryogenic calli from young embryos as explants, bean chitinase gene was transferred into spring wheat plants by microprojectile bombardment.

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  • 幼胚诱导的愈伤组织,经潮霉素抗性筛选,得到可育的再生植株。

    The regenerated fertile plants were obtained by effective selection for hygromycin B resistance.

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  • 研表明花生幼胚离体培养可以发育为成熟胚,并初步建立了培养技术。

    The results showed that peanut young embryos can be induced to be a mature one in vitro culture.

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  • 极早熟桃品种“春蕾”果实硬熟期的幼胚在胚珠离体培养条件下维持胚性生长。

    The young embryo of super-early maturing peach variety 'Chunlei 'at fruit hard-ripe stage maintain embryonic growth under the conditions of in vitro culture of ovule.

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  • 但幼胚诱导的愈伤组织经固体继代培养和液体悬浮培养后,都没有胚状体的分化。

    But all the calli induced by immature embryos did not differentiate the embryoids through solid and liquid subculture.

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  • 酶蛋白的最大含量在原胚阶段初为20.83%,以后至幼胚阶段急剧减少到4.9%。

    Enzymic protein content decreases from the maximum at early phase of proembryo stage (20.83%) to the minimum at young embryo (4.9%).

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  • 利用PDS10 0 0 /氦气基因枪将水稻几丁质酶基因导入小麦幼胚盾片愈伤组织。

    Rice chitinase gene was transferred into immature embryogenic calli of wheat using PDS1000/He particle delivery system.

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  • 结果表明:空篷栗在幼胚发育期、子叶发育期,其总苞和子房中的蛋白质含量均低于正常栗;

    The results showed that in the developmental periods of young embryo and cotyledon, protein contents of ovary of empty shell chestnut were lower than that of normal chestnut;

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  • 本研究从优化玉米幼胚培养遗传转化受体系统和抗病毒基因pap的转化两个方面进行研究。

    Thus the suitable acceptance system of maize genetic transformation and transferring resistant maize virus gene (PAP) were studied.

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  • 结果表明春性小麦幼胚离体培养的最佳诱导培养基为L3D2,最佳分化培养基为L3D0 1zt10。

    The results showed that the optimized inducing and differentiation medium for tested cultivars are L3D2 and L3D0.1ZT10 respectively.

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  • 通过石蜡切片观察了转基因水稻幼胚的发育情况,结果表明细胞和组织的分化在胚的发育过程中被阻断了。

    The rice embryo development was investigated by paraffin section, and the results indicated that cell and tissue differentiation was interfered during embryo development.

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  • 优化了小麦组织培养条件,建立了以小麦幼穗切段、幼胚盾片和成熟胚盾片为外植体的高效稳定再生体系。

    The high efficient plant regeneration systems have been established by optimizing tissue culture conditions using inflorescence explants, immature and mature embryo scutella as the target materials.

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  • 从对酸铝敏感的栽培大麦品种“早熟3号”的花药和幼胚培养物中,分别诱导了单倍体和二倍体愈伤组织。

    The haploid and diploid calli were induced respectively from anther and immature em- broy cultures in barley cultivar'Zhaoshu No. 3', which is susceptible to acid-aluminum.

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  • 小麦基因型扬麦158、扬麦10是优良的转化材料,以预培养4天的小麦幼胚为受体,培养力高,再生能力强。

    Wheat genotypes Yangmai 158, Yangmai 10 are excellent transformation materials. Wheat immature embryos with 4 days preculture have high tissue culture response and strong regeneration capacity.

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  • 以4个小麦品种的幼胚为外植体,通过脱分化和再分化培养,研究了影响小麦幼胚愈伤组织诱导和再分化的因素。

    The explants from immature embryo of 4 wheat varieties were cultured in vitro to study the affecting factors on callus induction and differentiation.

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  • 作者利用成单玉米15,18号的幼胚诱导的愈伤组织,系统地研究了愈伤组织的改良、悬浮细胞系的建立及植株再生。

    The callus texture modification, establishment of suspension cell lines and plant regeneration were systematically studied with immature embryos from Chengdan15,18 genotypes in maize.

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  • 以该配方诱导幼胚,处于胚胎选择阶段和组织与器官分化阶段的幼胚,能诱导出松软、晶莹透明的愈伤,诱导频率为2-6%。

    With this medium, soft, transparent callus can be induced from immature embryos of embryo selection or tissue and organ differentiation stages. The induction frequency was 2-6%.

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  • 以玉米自交系4112的幼胚为外植体,以MS为基本培养基,研究了不同质量浓度的2,4 - D对愈伤组织诱导的影响。

    Influence of different mass concentration of 2, 4-d on callus induction in maize inbred line 4112 from immature embryos with MS being used as basal culture medium was studied.

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  • 合成后的新细胞自动按照植入DNA序列“重新编程”,直到胚胎进入囊胚期,即由50至100个细胞组成的一个中空的球形幼胚。

    The egg automatically 'reprogrammed' its DNA until it reached a stage of the embryo known as a blastocyst — a hollow ball of 50 to 100 cells.

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  • 结果表明,幼胚和成熟胚的愈伤组织诱导率无明显差异,但较高浓度的2,4-D有利于成熟胚的诱导,而幼胚培养时2,4-D浓度的影响效果因品种而异;

    The results showed that there were no difference in callus induction between two kinds of explant. High concentration of 2,4-D was effective on the mature embryos' induction.

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  • 野牛草组织培养的外植体有幼苗片段、叶基、雄花幼穗和成熟胚等。

    In Buffallograss tissue culture, seedling segment, leaf base, mature embryo and immature male inflorescences can be used as explant.

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  • 将646枚2 ~4细胞期重构胚移植到38只同期化及非同期化的受体后无幼仔出生。

    When 646 reconstructive embryos in 2-4 cells were transferred into 38 recipients under synchronous and asynchronous, no child was born.

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  • 将646枚2 ~4细胞期重构胚移植到38只同期化及非同期化的受体后无幼仔出生。

    When 646 reconstructive embryos in 2-4 cells were transferred into 38 recipients under synchronous and asynchronous, no child was born.

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