结果1．单管巢式PCR反应条件中的模板量及外引物循环数对检测结果的影响较大。

Result (1)It is affected to the PCR results by the quantities of templates and outer cycles in one tube nested PCR.

youdao

用巢式PCR、引物延伸预扩增法扩增单细胞，扩增率分别为90％和85％。

Amplification rate of nest-PCR and PEP was 90% and 85%, respectively.

youdao

用巢式PCR、引物延伸预扩增法扩增单细胞，扩增率分别为90％和85％。

Amplification rate of nest-PCR and PEP was 90% and 85%, respectively.

youdao