酿酒酵母系统是最重要的外源基因表达系统之一。
Saccharomyces cerevisiae is one of the most important heterologous expression systems.
甲醇营养型酵母和裂殖酵母作为外源基因表达的有效系统，正引起人们广泛研究。
Methlotrophic yeast and fission yeast S. pombe have received attention as a good foreign genes expression system.
这些作物在大田种植过程中以及收获后，其植物残体、根系分泌物和飘落的花粉等不断地进入土壤，可能造成外源基因表达蛋白在土壤中积累。
Transgenic resistant-pest plants can release transgene proteins through their residues, root exudates and pollens, and these proteins are bound rapidly to soil active particles and persisted in soil.
采用间接免疫荧光法(IFA)检测外源基因的表达。
The expression of exogenous genes were analyzed by indirect immunofluorescence assay (IFA).
采用间接免疫荧光法(IFA)检测外源基因的表达。
The exogenous gene expression was analyzed by indirect immunofluorescence assay (IFA).
这些质粒可用于外源基因在酵母中表达的研究。
These plasmids can be used in the study of foreign gene expression in yeast.
外源基因在质体中的表达调控受多种因素影响。
Regulation of foreign gene expression in plastids is influenced by various factors.
实验结果表明，以TK基因构建的重组载体质粒可用于外源基因的重组表达研究。
The result showed that the transfer vector plasmid constructed with FPVTK genes can be used to express foreign gene in FPV recombinant.
转化的外源基因在植物受体组织中能否正确、高效并按照人们的意愿特异地表达是人们非常关注的问题。
Whether the exogenous gene of the conversion can be expressed correctly, efficiently and as desired in the plant recipient is of current concern.
GUS活性检测和胭脂碱分析表明，外源基因在转化细胞内得到稳定表达。
Enzyme activity of GUS and NOS assay demonstrated that foreign genes expressed in the transformed plants and calli.
基因治疗的成功有赖于安全有效的基因转移方法和高水平表达的外源基因。
Successful gene therapy relies on safe and efficient gene transfer methods and high level expression of foreign gene.
结果表明外源激素促进了该基因的表达。
The results are as follows: Exogenous hormones enhance HFT gene expression.
作者简述了利用植物生产外源蛋白的主要策略及异源基因在植物中表达的研究进展。
A brief review of major strategies for the production of heterologous proteins and the expression of foreign genes in plants is introduced in this article.
结论：外源质粒dna通过胃肠道途径可广泛调控肠道多种基因表达。
CONCLUSION: Foreign plasmid DNA, administered via the gastrointestinal tract, may widely modulate the expression of many genes in the intestine in mice.
目的探讨胚胎大鼠神经干细胞的体外培养条件及外源基因的表达效率。
Objective to explore the culture conditions for the neural stem cells from embryonic rat cortices and to test their efficacy of expressing exogenous gene.
结论：可通过四环素抗性筛选系统筛选外源基因的高效表达克隆。
Conclussion: We could screen high expressed clone of heterologous gene using tetracycline resistance screening system.
外源基因在大肠杆菌染色体上的稳定表达并不影响细菌的生长繁殖。
The stable expression of exogenous gene in E. coli chromosome had no effect on the bacterial growth and propagation.
外源基因在该基因工程菌中得到了高效表达。
The gene was highly expressed in the engineering bacterial strain.
结果表明，人工转录因子能有效地促进外源基因在哺乳动物细胞中的表达。
These results indicated that artificial transcription factor is potent in the enhancement of exogenous gene expression in mammalian cells.
为满足转目的基因的需求，探索一种能够将外源基因转入银杏细胞，并得以稳定表达和遗传的转基因方法以及选择合适的载体。
To meet the demands of gene transfer of an objective gene, a suitable vector should be screened to carry the extraneous objective gene into the callus cell of Ginkgo biloba.
说明外源BADH基因可提高小麦中甜菜碱含量，但在不同品系中的表达有一定差异。
It was indicated that the foreign BADH gene could improve the betaine content in wheat, but the improvements were not the same in different stains.
外源基因在大肠杆菌中高效表达时，通常会形成不溶性、无活性的蛋白聚集体——包涵体。
Over expression of recombinant proteins in Escherichia coli cytoplasm often results in the formation of insoluble inclusion bodies.
为构建其启动外源基因的质粒表达载体作准备。
It was therefore preparation for constructing an expression plasmid vector to express exogenous genes.
GUS染色证明外源目的基因在玉米细胞和组织中得到了表达。
GUS assay indicated that foreign gene had expressed in maize cell and calli.
目的获取番茄果实特异性e8启动子基因，为实现外源基因在转基因番茄中果实特异性表达做准备。
Objective to obtain the gene encoding tomato fruit-specific E8 promoter therefore to prepare for exogenous gene transcription and expression in transgenic tomato fruit.
为从基因水平上改造腈水合酶，进行了诺卡氏菌腈水合酶基因的外源表达研究。
Heterogenous expression of active nitrile hydratase (NHase) was focused for its great potential in genetically evolution of the operational stability of NHase.
结果剔除毕赤酵母中的PEP4基因后外源蛋白质表达量平均提高9．5%。
Results By disrupting the PEP4 gene in Pichia pastoris, the productivity of heterologous protein expression increased 9.5% on average.
此设计方案不仅大大提高了外源基因的表达量，且极大地降低了筛选阳性克隆的劳动强度。
This project not only increased the expression level of the exogenous gene, but also drastically reduced the labor strength of screening positive clones.
外源基因的片段大小及连接方式对其表达也有一定影响。
The sizes and link styles of exogenous genes also had some effects on their expression quantities.
外源基因的片段大小及连接方式对其表达也有一定影响。
The sizes and link styles of exogenous genes also had some effects on their expression quantities.
应用推荐