发酵液经过预处理后，依次用大孔树脂吸附，硅胶柱吸附层析和薄层层析等分离方法即可获得农用抗生素TS99纯品。
Pure TS99 could be obtained from pretreatment fermentation broth through macroreticular adsorbent, silica gel column chromatography and thin-layer chromatography.
混合模式吸附层析是一种新型的生物分离方法，其功能配基兼有两种或两种以上的作用模式，主要为静电和疏水相互作用。
Mixed-mode chromatography is a new kind of chromatography technology for bioseparation. The special ligand combines two or more interaction modes, commonly hydrophobic and electrostatic interactions.
论文最后对柱层析的有关吸附等温线理论进行了探讨。
The related theory of adsorptive isotherm was studied about columniation layer analyse in the last of thesis.
通过对6种大孔树脂的静态吸附和动态吸附试验，选择HPD600和HPD450两种大孔树脂做柱层析。
Two resins, HPD600 and HPD450, were selected among 6 different macroporous absorption resins after static state and dynamic state absorbance.
本文结合亲硫层析和混合模式吸附，制备新型的亲硫性混合模式吸附剂，并用于抗体分离。
Combining the thiophilic chromatography and mixed mode adsorption, new adsorbent with thiophilic mixed-mode ligands were developed and used for antibody separation.
研究了通过大孔吸附树脂纯化苹果中原花青素柱层析的最佳条件。
We studied the optimum conditions for separating procyanidins from apple juice by macroporous resin.
研究了离子沉淀法和大孔吸附树脂层析法结合制备茶多酚的工艺。
Combining the ion precipitation with column chromatography to prepare tea polyphenol was studied.
方法通过改变层析条件吸附剂、展开剂、 显色剂，反复实验，筛选出适宜的层析条件。
Methods To select best chromatographic condition by trial and error, making changes in sorbent, developing agent.
综合利用吸附法、溶媒萃取法和硅胶柱层析法对抗生素进行了纯化。
Finally, the antibiotics were purified by adsorption, solvent extraction and silica gel column chromatography.
筛选出了柱层析吸附剂，通过柱层析最终得到精制卵磷脂。
Using this salt and adsorbent, the flax lecithin was refined.
热力学数据表明它们在层析硅胶上的吸附属于自发吸附过程。
Thermodynamic data shows that their adsorption on chromatography silica gel is spontaneous.
方法：将皱瘤海鞘的水溶性部分上大孔吸附树脂柱层析，用不同浓度的乙醇溶液洗脱，将样品进行初步分离。
Methods Using water and ethanol with different concentration, Water-soluble substance of Styela. Plicata was separated. Results Water-soluble substance of Styela. Plicata was separated into six parts.
发酵产物经大孔吸附树脂、硅胶柱层析及重结晶等方法制备出牛蒡子苷元纯品，并采用核磁共振（NMR）、红外（IR）、质谱（MS）等方法对产物进行了鉴定。
The product of fermentation was separated with macroporous resins, and then purified by the silica-gel column chromatography and recrystallization. The pure product was assayed by NMR, IR and MS.
结果表明最佳条件为：柱层析的吸附剂为：100-200目层析硅胶；
As a result; the optimum conditions of Lycopene were obtained. Absorbent: 100~200 silica gel;
结果表明最佳条件为：柱层析的吸附剂为：100-200目层析硅胶；
As a result; the optimum conditions of Lycopene were obtained. Absorbent: 100~200 silica gel;
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