目的通过细菌内同源重组方法构建重组腺病毒。
Objective To construct recombinant adenovirus by homologous recombination in bacteria.
我们以这些片段开始，然后通过同源重组将其组合到染色体中。
We start with these fragments, and then we have a homologous recombination system that reassembles those into a chromosome.
经同源重组后，以终点稀释和斑点杂交法筛选重组杆状病毒；
After co-transfection of recombinant plasmid and wild type AcNPV DNA, the rAcNPV was confirmed by the end point dilution assay and dot blot.
负向选择系统的应用使同源重组事件的富集效率提高了7倍。
The use of negative selection gene (HSV-tk) results in 7-fold increase at selection efficiency.
更新较缓慢的组织中，似应首选同源重组，因其干细胞不需要种植。
Homologous recombination would presumably be preferred in the case of more slowly-renewing tissues, whose stem cells should not need reseeding.
目的利用细菌内同源重组法构建含ICOS胞外区基因的重组腺病毒。
Objective To construct the recombinant adenovirus of human ICOS extracellular domain gene by using the method of homologous recombination in bacteria.
在同源重组中一条染色体的单链与另一条染色体的单链连接形成的结构。
Structure formed during homologous recombination connection of single strands of one chromosome to the other chromosome.
结果显示，MVA同源重组频率虽然比其他痘苗病毒株要低，但仍然是较高的。
The results showed that the recombination frequency of these rMVAs was significantly high, though lower than that observed in other vaccinia virus strains.
这项研究报告的一些重要证据表明A型流感病毒同源重组在人类和猪的谱系中发生。
This study reports several significant evidence demonstrating that homologous recombination also takes place between influenza a viruses in human and swine lineages.
基因打靶技术是建立在胚胎干细胞和同源重组技术之上，可对基因组进行定点修饰的实验方法。
Established upon the embryo stem cell technique and homologous recombination, gene targeting has been widely used in the genome specific manipulation.
同源重组的后果，在不同等位基因之间的杂交使其中的一个等位基因被转换成了另一个等位基因。
Consequence of homologous recombination, in which hybridization between different alleles causes one allele to be converted into the other.
大多数断裂在同源重组（HR）期间可快速而准确固定，它们可使用DNA完整拷贝作为修复模板。
Most breaks are quickly and accurately fixed during the process of homologous recombination (HR), which uses an intact copy of DNA as a template for repair.
结果获得了经非同源重组整合到酵母染色体上的能有效利用甲醇作为唯一碳源的PMD11和PMD16转化株。
Results the PMD11 and PMD16 transformants were obtained, capable of metabolizing methanol as sole carbon resource through non homologous integration into the yeast chromosome.
本发明还涉及人源化改造的基因突变技 术和去除GFP的腺病毒转移载体及去除GFP的同源重组腺病毒载体。
The invention also relates to a human reforming gene mutation technique, adenovirus transition carrier removing GFP and homologous recombinant adenovirus carrier.
同源重组的后果，在不同等位基因之间的杂交使其中的一个等位基因被转换成了另一个等位基因。这是由错配修复引起的。
Consequence of homologous recombination, in which hybridization between different alleles causes one allele to be converted into the other. This occurs by mismatch repair.
目前已鉴定的这一基因家族的多数成员均参与几种重要的DNA修复途径，包括碱基切除修复、同源重组修复和非同源末端重接。
Most members of the XRCC genes family participated in several DNA repair pathways, including base excision repair, homologous recombination repair and non-homologous end joining.
这意味着减数分裂中同源染色体不能配对，染色体间形成的交叉也会更少（例如，在减数分裂早期的重组位点）。
This means that homologous chromosomes failed to pair together during meiosis and chromosomes formed fewer crossovers, i. e. sites where they recombine, during the initial stages of meiosis.
方法采用同源基因重组技术。
农抗5102产生菌同源菌株9011和FR008的原生质体融合重组子SR7能产生1种两亲本所没有的抗细菌活性物质。
The fusion recombinant SR 7 of the antibiotic 5102 producing homogenous strains 90 11 and FR 008 produced a new antibacterial substance the parents could not.
结果结核分支杆菌重组抗原esat - 6与已发表氨基酸序列同源性为90%。
Results Compared with the amino acid sequence of ESAT-6 of Mycobacterium tuberculosis published, the homologies was 90%.
结果结核分支杆菌重组抗原esat - 6与已发表氨基酸序列同源性为90%。
Results Compared with the amino acid sequence of ESAT-6 of Mycobacterium tuberculosis published, the homologies was 90%.
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