来自受体菌自身的同源质粒,因克服了宿主的限制一修饰性,可以极显著地提高电转化效率。
A significant increase of transformation efficiency was revcaled with the homologous plasmid isolated from recipient itself since the effects of host restriction and modification werc avoided.
方法用计算机同源模建预测多肽的抗原性,然后用淋巴细胞转化试验验证其抗原性。
Methods the antigenicity of the synthetic peptide were predicted by the computer modeling, then verified by lymphocyte transformation test.
结果获得了经非同源重组整合到酵母染色体上的能有效利用甲醇作为唯一碳源的PMD11和PMD16转化株。
Results the PMD11 and PMD16 transformants were obtained, capable of metabolizing methanol as sole carbon resource through non homologous integration into the yeast chromosome.
序列分析和突变研究表明,这些去卤化酶相对那些可转化非卤化底物的酶是同源的。
Sequence analysis and mutagenesis studies indicate that several dehalogenases are homologous to enzymes that carry out transformations on non...
糖多孢红霉菌的原生质体转化和染色体同源整合,是红霉素生物合成基因改造的重要途径。
Protoplast transformation and homologous chromosomal integration of s erythraea was an important path in changing gene involved in erythromycin biosynthesis.
糖多孢红霉菌的原生质体转化和染色体同源整合,是红霉素生物合成基因改造的重要途径。
Protoplast transformation and homologous chromosomal integration of s erythraea was an important path in changing gene involved in erythromycin biosynthesis.
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