此外我们还用同源克隆策略获得了其它物种的ARHT2基因。
We get the ARHT2 genes of other species by homologous clone strategy.
采用同源克隆及基因组步移的方法,分离克隆了牙鲆肌肉生长抑制素(MSTN)基因。
Myostatin(MSTN)gene was isolated from Japanese flounder(Paralichthys olivaceus)by homology cloning strategy and genome walking.
本研究以果蔗为实验材料,利用同源克隆技术克隆了ACO基因,并对该基因做了相关研究。
In this study, using chewing cane as experimental materials, cloned ACO gene by homology cloning techniques and made some other researches on it.
猴hcbp6同源基因的克隆化为研究不同种属来源的HCBP6基因的结构域功能、表达与调控等研究奠定了基础。
The successful cloning of monkey HCBP6 gene paves a way for the study of the relationship of structure and function of HCBP6 genes from different species.
目的:建立一套稳定可靠的方法,对过敏原性物种中的过敏原同源基因进行快速克隆。
AIM: To establish a stable and reliable method for fast cloning homologous genes of pollen allergens in allergen-containing plants.
结果:随机挑选的16个克隆中,有8个与人CD59的结合力高。测序后,得到3个高度同源的氨基酸序列。
RESULTS: 8 out of 16 phage clones which were chosen randomly were identified to have high binding power to CD59. After sequencing, 3 high homologous amino acids were obtained.
目的利用同源性分析,克隆定位新的人类间隙连接蛋白基因,并探讨该基因和遗传性耳聋的关系。
Objective To clone a novel human connexin gene and find out the relationship between this gene and hereditary deafness.
结论利用同源序列克隆进化上保守的基因是简捷快速的方法。
Conclusion It is a simple and rapid method to clone gene through homologene sequence.
根据同一个体不同寡克隆CDR3区的同源性,这些寡克隆可分为不相关(54.5%)、部分相关(27.3%)和完全相关(18.2%)。
And the oligoclones could be classified into not related(54.5%), partially related(27.3%)and completely related(18.2%)according to homology of CDR3sequence in an individual patient.
表明根据物种间同源基因序列,对跨物种间EST数据库进行同源检索、筛选、拼接,是克隆基因的有效途径。
The results showed that it was an efficient technique to clone new genes by searching EST database with homologous gene of model species.
结果克隆了PPO基因,找到了PPO基因与小鼠、果蝇、蚊子、线虫以及酵母等的同源基因,比较发现PPO在进化上非常保守。
Results The PPO gene was cloned and its counterparts in mouse, drosophila, anopheles Gambia and yeast were found by database search.
说明根据物种间同源基因序列,进行跨物种EST数据库的同源检索筛选、拼接,是基因克隆的一条有效途径。
The results show that it is a convenient technique for cloning new gene by searching EST database with homologous gene of model species such as fruit fly.
运用基于基因组数据库的特定基因同源新基因的克隆策略得到一个人类新基因WDR70,该基因编码一个包含12个WD 4 0结构域的蛋白。
A human novel gene WDR70 is obtained with the cloning strategy of novel homologous gene, which is a specific gene based on genome database. It encodes a protein of 12 WD40 domain.
结果挑选了4个差异筛选的阳性克隆进行测序,序列同源性分析表明它们均与胰岛素-i基因序列高度一致。
Results four positive clones of differential screening were picked out for sequencing. Homology analysis indicated that all of the four clone sequences were the same as that of insulin-I gene.
挑选阳性克隆进行测序并用BLAST软件进行同源性分析。
The positive clones were sequenced, and the sequences were analyzed by the BLAST program for homology search.
所克隆的奶山羊blg基因调控区与山羊blg伪基因显著不同,其同源性仅为83 %(上游调控区)和88%(下游调控区)。
The dairy goat gene differed significantly from goat BLG pseudo gene with a homology of only 83% (upstream region) and 88% (downstream region), respectively.
通过序列同源比对及系统发育进化分析,发现克隆到的序列与其它物种DOB基因具有很高的同源性。
Sequence of SLA-DOB1 was cloned that was high homology with the other species by multiple sequences alignment and phylogeny evolution analysis.
通过序列同源比对及系统发育进化分析,发现克隆到的序列与其它物种DOB基因具有很高的同源性。
Sequence of SLA-DOB1 was cloned that was high homology with the other species by multiple sequences alignment and phylogeny evolution analysis.
应用推荐