• 此外我们还用同源克隆策略获得其它物种ARHT2基因

    We get the ARHT2 genes of other species by homologous clone strategy.

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  • 采用同源克隆基因组步移的方法,分离克隆了牙鲆肌肉生长抑制素(MSTN基因

    Myostatin(MSTN)gene was isolated from Japanese flounder(Paralichthys olivaceus)by homology cloning strategy and genome walking.

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  • 研究果蔗实验材料利用同源克隆技术克隆ACO基因基因相关研究。

    In this study, using chewing cane as experimental materials, cloned ACO gene by homology cloning techniques and made some other researches on it.

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  • hcbp6同源基因克隆化为研究不同种属来源的HCBP6基因结构功能、表达调控等研究奠定了基础。

    The successful cloning of monkey HCBP6 gene paves a way for the study of the relationship of structure and function of HCBP6 genes from different species.

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  • 目的建立一套稳定可靠方法过敏原性物种中的过敏原同源基因进行快速克隆

    AIM: To establish a stable and reliable method for fast cloning homologous genes of pollen allergens in allergen-containing plants.

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  • 结果随机挑选16克隆中,8个与人CD59的结合力测序得到3个高度同源氨基酸序列。

    RESULTS: 8 out of 16 phage clones which were chosen randomly were identified to have high binding power to CD59. After sequencing, 3 high homologous amino acids were obtained.

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  • 目的利用同源性分析,克隆定位新的人类间隙连接蛋白基因探讨基因遗传性耳聋关系

    Objective To clone a novel human connexin gene and find out the relationship between this gene and hereditary deafness.

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  • 结论利用同源序列克隆进化上保守的基因简捷快速方法

    Conclusion It is a simple and rapid method to clone gene through homologene sequence.

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  • 根据同一个体不同寡克隆CDR3区的同源性,这些克隆分为相关(54.5%)、部分相关(27.3%)和完全相关(18.2%)。

    And the oligoclones could be classified into not related(54.5%), partially related(27.3%)and completely related(18.2%)according to homology of CDR3sequence in an individual patient.

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  • 表明根据物种同源基因序列,对跨物种间EST数据库进行同源检索、筛选、拼接,克隆基因有效途径

    The results showed that it was an efficient technique to clone new genes by searching EST database with homologous gene of model species.

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  • 结果克隆PPO基因,找到了PPO基因小鼠果蝇、蚊子、线虫以及酵母等同源基因,比较发现PPO进化上非常保守。

    Results The PPO gene was cloned and its counterparts in mouse, drosophila, anopheles Gambia and yeast were found by database search.

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  • 说明根据物种同源基因序列,进行跨物种EST数据库同源检索筛选、拼接,基因克隆一条有效途径。

    The results show that it is a convenient technique for cloning new gene by searching EST database with homologous gene of model species such as fruit fly.

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  • 运用基于基因组数据库特定基因同源新基因克隆策略得到人类新基因WDR70基因编码一个包含12WD 4 0结构蛋白

    A human novel gene WDR70 is obtained with the cloning strategy of novel homologous gene, which is a specific gene based on genome database. It encodes a protein of 12 WD40 domain.

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  • 结果挑选4个差异筛选阳性克隆进行测序,序列同源分析表明它们均与胰岛素-i基因序列高度一致

    Results four positive clones of differential screening were picked out for sequencing. Homology analysis indicated that all of the four clone sequences were the same as that of insulin-I gene.

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  • 挑选阳性克隆进行测序并用BLAST软件进行同源分析

    The positive clones were sequenced, and the sequences were analyzed by the BLAST program for homology search.

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  • 克隆奶山羊blg基因调控山羊blg基因显著不同同源为83 %(上游调控)88%(下游调控区)。

    The dairy goat gene differed significantly from goat BLG pseudo gene with a homology of only 83% (upstream region) and 88% (downstream region), respectively.

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  • 通过序列同源比对及系统发育进化分析,发现克隆序列其它物种DOB基因具有很高同源性。

    Sequence of SLA-DOB1 was cloned that was high homology with the other species by multiple sequences alignment and phylogeny evolution analysis.

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  • 通过序列同源比对及系统发育进化分析,发现克隆序列其它物种DOB基因具有很高同源性。

    Sequence of SLA-DOB1 was cloned that was high homology with the other species by multiple sequences alignment and phylogeny evolution analysis.

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