采集安徽和四川两省的十二指肠钩虫，用PCR技术扩增COI基因，同时测序并用MEGA软件进行遗传差异的分析。
COI genes of hookworm was amplified from hookworm genomic DNA by PCR and sequenced. Program MEGA was used for the sequence analysis.
采用同时蒸馏微萃取法提取姜科植物生姜中挥发油，并用气相色谱-质谱联用技术对其挥发性化学成分进行分析。
The volatile oil from ginger was extracted by the method of simultaneous distillation microextraction, the components were separated by gas chromatography and identified by mass-spectrometry.
方法用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化，并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白（GFAP）和增殖细胞核抗原（PCNA ）的表达水平。
Methods We measured the astrocyte cell cycles in different time after ischemia and anoxia by flow cytometry and detected the levels of GFAP and PCNA with fluorescence immunocytochemistry.
方法用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化，并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白（GFAP）和增殖细胞核抗原（PCNA ）的表达水平。
Methods We measured the astrocyte cell cycles in different time after ischemia and anoxia by flow cytometry and detected the levels of GFAP and PCNA with fluorescence immunocytochemistry.
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