具有原代细胞生物形态学特征。
The cells possessed the morphological characteristics as the primary ones.
原代细胞来自生物体本身。
组织块培养法对于原代细胞的培养要优于酶消化法。
The method of explanted tissue culture is better than the method of enzyme digestion for the primary cells.
目的：探讨胰蛋白酶消化组织块制备原代细胞的最佳浓度。
Objective: to approach the optimal concentration of trypsin and the best method on the dissociation of the tissue to obtain separated primary cells.
最后，气管抽吸的原代细胞和之后的传代细胞有同样模式。
Finally, primary cells from tracheal aspirates behaved in an identical manner as later passage cells.
结果全骨髓直接培养法原代细胞形态不均一，但是增殖能力好；
Results The primary cells of complete marrow direct culture method were uneven in shape, but with excellent reproductive activity;
目的：探讨白藜芦醇诱导人胃癌原代细胞裸鼠移植瘤凋亡的机制。
AIM: to investigate the molecular mechanism of the apoptosis of implanted tumor of human primary gastric cancer cells in nude mice induced by resveratrol.
低剂量组al原代细胞有凋亡和胀亡两种方式改变，以胀亡性改变为主。
AL primary cells of ATS low dose group had tow changes of apoptosis and oncosis which was the main.
他们将分化上晚于干细胞的原代细胞注射到能保持牙体结构的胶原架中。
They used primitive cells, not quite as early as stem cells, and injected them into a framework of collagen, the material that holds the body together.
结论：利用多次差速传代可从混合培养的原代细胞中获得纯化的牙囊细胞。
Conclusion: Dental follicle cells can be purified by several differential passages from the mixed primarily cultured cells.
方法结合酶消化法和组织块培养法，体外单层培养人退变纤维环原代细胞。
Method:Primary cells of human degenerative AF were obtained through in vitro monolayer culture combined with enzyme digestion method and tissue pieces culture method.
目的：研究乙醇对铁过载大鼠肝原代细胞脂质过氧化反应和胶原合成的影响。
Objective: The experiment was performed to characterize the effect of ethanol on peroxidation and collagen synthesis in primary hepatocyte culture of iron overloaded rats.
目的建立一种简单、易行、满足基本科研需要的脊髓神经元原代细胞分散培养法。
Objective To find an easy, practical and reliable method for dispersion culture of primary neurons in rat spinal cord.
目的研究肺癌原代细胞和细胞系a549对肿瘤浸润性淋巴细胞的抑制作用及其临床意义。
Objective To study the inhibition of lung cancer cells of primary culture and A549 cell line on the proliferation of tumor-infiltrating lymphocytes (TIL) in vitro and its clinical implications.
选用新生SD大鼠的髁状突软骨作为原代细胞培养的组织来源，建立髁状突软骨细胞有限细胞系；
The primary MCC separated from the mandibular condylar cartilage of six neonatal SD rats were cultured and a finite cell line was established in vitro.
结果两种方法均能得到乳腺癌原代细胞，组织块培养法的成功率为90.0%，细胞培养法的成功率为20.0%。
Results the breast carcinoma cells could be cultured by these two methods with a success rate of 90% in the tissue culture method and that of 20% in the cell culture method.
结论：研究提示青蒿琥酯抗急性白血病原代细胞的作用机制之一是通过降低其线粒体膜电位，进而使其胞内环境改变而使肿瘤细胞死亡。
One of its mechanisms is through reduces the cell line plastochondria membrane potential, then causes in its butcher the environment change to cause the tumour cell death.
目的探讨凝血酶对体外培养的原代大鼠脑微血管内皮细胞(bmvec)的损伤作用及凝血酶抑制剂水蛭素的保护作用。
AIM to investigate the apoptotic effect of thrombin on brain microvascular endothelial cell (BMVEC) of cultured rat, and the protective effect of hirudin, an inhibitor of thrombin.
迄今为止我们已取得了突变的原代培养的细胞以及蠕虫”。
To date we have made mutant tissue-cultured cells as well as worms.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
目的筛选一种动物原代敏感细胞用于彗星实验，以便更广泛地监测环境样品的遗传毒性。
Objective in order to select sensitive primary cells to be used in the comet assay to detect genotoxicity for environmental samples.
目的对大肠腺瘤细胞原代培养的方法作进一步的改进。
Objective To improve the primary cell culture methods of colorectal adenomas.
目的：探索人脂肪源性基质细胞原代培养方法。
Objective: To establish a primary human adipose-derived stromal cells culture method.
方法以人脐静脉内皮细胞（HUVEC）原代培养模型，MTT法测定紫杉醇对HUVEC增殖的影响；
Methods Human umbilical vein endothelial cells were cultured primarily in vitro and influence of Paclitaxel on HUVEC proliferation was evaluated by MTT method.
结果：原代培养的RPE细胞含大量色素呈多角形，传代的细胞透明呈梭形。
Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape.
前言：目的：研究姜黄素在体外对原代白血病细胞的抑制作用。
Objective: To investigate the effects of curcumin on human acute myelocytic leukemia cells in vitro.
原代白血病细胞的再增殖能力低于白血病细胞系。
The regrowth ability of primary leukemia cells was lower than leukemia cell line.
方法：牛晶体上皮细胞原代培养。
Methods: Bovine lens epithelial cells (BLEC) were cultured in vitro.
用组织块直接培养法，也可以得到皮肤的原代培养细胞。
The primary culture cells of rabbit skin tissue could be obtained by explant culture too.
结论添加晶体皮质可提高晶体上皮细胞原代培养成功率。
ConclusionAdding lens cortex can increase the success rate of primary culture of lens epithelial cells.
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