MTT法检测加入反义寡核苷酸后对转染的包装细胞生长的影响。
MTT was used to detect the cell growth of the packaging cells.
结果多聚腺苷酸化信号缺陷病毒可以在PA317病毒包装细胞形成病毒颗粒；
Results polyadenylation signal-deficient retroviruses could be packaged by PA317 packaging cells.
构建并鉴定携带人NT - 3基因的逆转录病毒载体以及稳定的包装细胞系。
Objective:To construct a recombinant defective retroviral vector carrying hNT3 gene and a stable virus-producing packaging cell lines , and then to identify them.
目的探讨利用荧光激活细胞分选技术获得有效重组逆转录病毒包装细胞系的方法。
Objective To develop a method for acquisition of efficient and stable retroviral packaging cells on the basis of fluorescence-activated cell sorting (FACS) technique.
我们将对此重组质粒在包装细胞（PT67）的扩增以及对靶细胞转导的效应作进一步研究。
The proliferation of recombinant plasmid in packaging cells (PT67) would be studied further as well as the transducing effect to the target cells.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
在正常细胞中，通过组蛋白标记或DNA包装蛋白，在泛素分子的作用下，BRCA1蛋白使这些区域保持沉默。
In normal cells, the BRCA1 protein keeps these regions silent by tagging histones, or DNA packaging proteins, with a molecule called ubiquitin.
组蛋白组成蛋白线轴，DNA缠绕在它的周围，使DNA紧密地包装在细胞核中。
Histones make up the spool of proteins around which DNA winds so that it is packaged compactly in the nucleus.
在高尔基复合体中修饰，包装后输出细胞或传递到细胞质中的其他场所。
Within the Golgi complex molecules are modified and packaged for export out of the cell or for delivery else where in the cytoplasm.
然而这是罕见的，而且需要两种流感病毒进入同一个细胞中，复制然后在包装过程中其产物混合。
That's a rare event, however, and requires that two flu strains invade a single cell, replicate and then get their products mixed up in the packaging.
各膜来单独包装在无菌细胞培养皿。
Each membrane comes individually packaged in a sterile cell culture dish.
这个新研究表明，DNA数量的错误和它在癌细胞里面如何包装-叫做非整倍体-可能影响免疫系统的能力来毁坏肿瘤细胞。
The new study suggests that errors in the quantity of DNA and how it's packaged inside cancer cells - called aneuploidy - might affect the immune system's ability to destroy the cells.
如巧克力和谷类食品的酒吧早已包装用流式细胞包装技术。
Foods such as chocolate and cereal bars have long been packaged using flow wrapping technology.
表明，UL34基因对病毒的早期包装、成熟和出芽以及对UL31蛋白和核纤层蛋白在感染细胞中的正确定位都具有重要作用。
Then, the important roles of the gene in viral primary envelopment, mature and budding, proper location of the UL31 protein and lamin protein in cells infected with the virus were pointed out.
SWI /SNF通过改变细胞核内的遗传物质包装来工作。
SWI/SNF works by altering the packaging of genetic material inside a cell's nucleus.
包装成逆转录病毒，感染小鼠肝癌细胞MM 45t。
The retrovirus carrying IL-18 gene was used to infect murine liver cancer cell MM45T.
经包装获得较高滴度的病毒上清，以共培养的方式感染5-氟尿嘧啶预刺激的小鼠骨髓细胞。
FACS analysis demonstrated that about 25% of murine 5 FU prestimulated bone marrow cells could be infected after cocultivation with producing cells in vitro .
经包装获得较高滴度的病毒上清，以共培养的方式感染5-氟尿嘧啶预刺激的小鼠骨髓细胞。
FACS analysis demonstrated that about 25% of murine 5 FU prestimulated bone marrow cells could be infected after cocultivation with producing cells in vitro .
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