概要介绍了单分子荧光检测的原理、方法及其在生命科学中的应用。
This review focuses on the principle, method and biological applications of single molecule detection based on fluorescence.
树枝状高分子荧光受组分比、代数、溶液浓度、溶剂等因素的影响。
Five generation of polyamide dendrimers with peripheral fluorophore are synthesized and characterized by IR, UV, etc.
建立了单分子荧光显微装置，利用分子梳方法实现了对DNA分子的拉伸。
DNA stretching was performed with molecular combing method. Metallic ion effects on DNA stretching were investigated.
观测了五种典型的电子束泵浦高压混合气体产生的卤惰三原子准分子荧光发射谱。
The fluorescence emission spectra of five typical triatomic rare gas halide excimers have been observed from electron-beam-excited high-pressure mixtures.
介绍用激光诱发分子荧光法探测流动液体中单个染料分子的基本原理和实验方法。
The concept and experimental method of single-molecule detection based on laser-induced fluorescence in liquid fluid have been described.
通过药物在该体系中的紫外吸收光谱及分子荧光光谱的变化，验证其萃取药物的机理。
Through the comparison of different conditions and research on the ultraviolet and fluorescence spectra of drugs, the extraction mechanism of drugs was deduced.
本文简介近几年单分子荧光检测研究的若干典型实例，以此展示这项技术的特点、优势及其可能的应用。
We present some typical systems studied by single molecule fluorescence detection to demonstrate its feature, advantage, and application.
特异性的小分子荧光探针以其体积小、膜透性好、背景噪音低以及制备方便的优点成为蛋白质研究的一个有力工具。
Site-specific small-molecule fluorescent probe with advantages of small size, membrane permeability, low background and simple preparation will be a.
为了利用这种基质金属蛋白酶，钱博士将他的荧光染料和一些正电荷一同连接在一个小的发夹形状的蛋白质分子的一端。
To make use of the MMPs, Dr. Tsien attaches his fluorescent dye, along with some positive charges, to one side of a small hairpin-shaped protein molecule.
由于硅质纳米棒的高纵横比，使得附着于硅纳米棒上的染色分子对所探测捕获沙门氏菌具有更强的荧光着色性。
Due to the high aspect ratio nature of the silicon nanorods, dye molecules attached to the silicon nanorods produce an enhanced fluorescence upon capture and detection of Salmonella.
为了利用这种基质金属蛋白酶，钱博士将他的荧光染料和一些正电荷一同连接在一个小的发夹形状的蛋白质分子的一端。
To make use of the MMPs Dr Tsien attaches his fluorescent dye, along with some positive charges, to one side of a small hairpin-shaped protein molecule.
这项技术使用两种类型的抗体，分别把它们附着在一个荧光染料分子上。
The technique USES two types of antibody, each attached to a fluorescent dye molecule.
分子必须进行多次复制(这一步被称为扩增)同时进行荧光示踪标记。
The molecule must be replicated many times (a step called amplification) and also labelled with fluorescent tags.
为判别这种光束是否可以用来分辨分子手性，他们将光束照射到荧光手性分子上，分子被激发后会发出荧光。
To see whether it could be used to distinguish between left and right-handed molecules, they shone it on fluorescent chiral molecules, which glow when they are excited.
图4，格里高利博士的另一幅作品：用荧光微生物绘制出的DNA分子演示图。
Figure 4, Another of Dr. Gregory's images: an impression of the DNA molecule rendered using glowing microorganisms.
研究人员设计了一种分子，这些分子可以提前粘附在神经细胞上，并用荧光标记这种分子。
The researchers created a molecule that binds preferentially to nerve cells, and labelled it with a fluorescent tag.
最简单的发光反应需要一种叫做荧光素有机分子，在荧光素酶的帮助下，进行被氧气氧化的氧化反应。
The simplest light-producing reactions require an organic molecule, known as a luciferin, to be oxidised by oxygen, a process that is helped along by an enzyme known as luciferase.
前言：在人类细胞遗传学中，荧光分子技术的出现带来了一些染色体研究和诊断的新方法。
Introducetion: in human cytogenetics, the advent of fluorescence molecular techniques has brought forth new procedures for chromosome investigation and diagnosis.
他们将设计一个系统，可以将单个荧光分子精确置于单个纳米立方体。
They plan to design a system with individual fluorescent molecule placed precisely underneath a single nanocube.
接下来研究人员又用另一种荧光分子标记了正处于分化为过程，处于初级阶段并被招募转变为成熟神经细胞的干细胞。
The researchers then used another fluorescent molecule to tag all stem cells that were undergoing division in the process of staying "fresh" in case they were recruited to become mature nerve cells.
尽管绿色荧光蛋白是由238个氨基酸组成蛋白质，是一种微小的、疏水性膜渗透的荧光分子。
While GFP is a protein that consists of 238 amino acids, the new technique USES a small, hydrophobic, and membrane-permeable fluorescent molecule.
荧光物质分子被化学反应激发后发出的光被称为化学发光。
ChemiLuntinescence (CL) is the emission of light after excitation of a fluorescent molecule by a chemical reaction.
该方法应用荧光显微术使单个DNA分子成像，这些单个的DNA分子已经被分成有序的片断，因而被称为限制性内切酶的识别序列。
The method USES fluorescence microscopy to image individual DNA molecules that have been divided into orderly fragments by so-called restriction enzymes.
荧光分子标记方法是可选择的方案之一。
Then, the fluorescent label will become convenient and feasible method.
通过将荧光分子置于立方体角落的边上，便可实现更高速率的光子发射。
According to Akselrod, they can achieve even higherfluorescence rates by standing the molecules up on edge at the corners of thecube.
如果需要有更高的分辨率，就需要加入荧光染色分子使其起效。
If higher resolution is required, the fluorescence dye molecules come into play.
如果需要有更高的分辨率，就需要加入荧光染色分子使其起效。
If higher resolution is required, the fluorescence dye molecules come into play.
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