目的探讨凝胶过滤层析分离蛋白质的最佳实验效果。
Objective to find out the optimal condition for separation of the protein mixture by gel filtration chromatography.
以离子交换和凝胶过滤层析对梧桐花粉变应原进行分离和纯化。
Two major allergens PT 13 and PT 53 in platanus hispanica pollen were isolated and purified by ion exchange chromatography and gel filtration chromatography.
采用G - 25凝胶过滤层析法测定了乳清水解物及其超滤液的分子量分布范围。
The molecular weight (MW) distribution of whey protein hydrolysate was determined by G-25 gel chromatography.
研究了利用批量离子交换层析与凝胶过滤层析相结合的方法纯化类人胶原蛋白i的最佳条件。
The method of batch ion exchange chromatography combined with gel filtration chromatography to purify Human-like Collagen Biopolymer I was investigated.
方法:利用亲合层析及凝胶过滤层析从小鼠黑色素瘤中提取小鼠黑色素瘤凝集素(MMA)。
Methods: MMA was prepared with asialo-fetuin affinity and gel-filtration chromatography form murine melanoma.
方法采用凝胶过滤层析技术对针刺全血清进行分段分离,并将分离的血清经冷冻干燥制成干粉。
Method The acupuncture serum were separated by gel filtration with Sephadex G 75 and molecular standards.
本文利用多肽A合成粗品中目标肽与杂质分子量的不同,通过凝胶过滤层析法达到初步分离的目的。
According the molecular weight difference between the objectivepeptide and the impurities in Polypeptide A crude, the product waspurified by gel chromatography.
采用凝胶过滤层析对抗菌物质粗提物进行纯化,并对提取、纯化效果进行分析,表明纳豆菌能够产生多个组分的抗菌物质。
Extraction and purification effects were then analyzed, experimental results indicated that various anti-bacteria substances can be extracted from natto fungus.
菌体经过超声破碎后反复洗涤包涵体,融合蛋白纯度达到70%,进一步用离子交换层析和凝胶过滤层析纯化使其纯度达95%以上。
The purity of fusion protein was about 70% after ultrasonic decomposition and washing repeatedly, and reached above 95 % after purification by ion exchange and gel-filtration chromatography.
融合蛋白基因在巴斯德毕赤酵母中进行表达后通过阳离子交换层析、反向层析和凝胶过滤对表达产物进行了分离纯化。
HSA-AX15(R13K ) fusion protein was purified to homogeneity by cation exchange chromatography, re verse phase chromatography and gel filtration after expressed in pichia pastor is.
融合蛋白基因在巴斯德毕赤酵母中进行表达后通过阳离子交换层析、反向层析和凝胶过滤对表达产物进行了分离纯化。
HSA-AX15(R13K ) fusion protein was purified to homogeneity by cation exchange chromatography, re verse phase chromatography and gel filtration after expressed in pichia pastor is.
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