花生种子发育过程几丁质内切酶、外切酶与抗黄曲霉侵染的关系研究。
Study on relationships between endochitinase exochitinase and resistance to aspergillus flavus during seeds development of arachis hypogaea l.
突变位点经限制性内切酶分析证实。
Restriction enzyme site analysis was used to confirm the mutation.
结果表明，限制性内切酶的表达形式十分混乱。
The results reveal that its expression is rather disordered.
通过核酸内切酶酶切分析及测序鉴定载体结构。
The structure of vector was identified by endonuclease analyzing and base sequencing.
经限制性内切酶反应，获得两个预期大小的片段。
After a restriction endonuclease cleavage, two expected smaller fragments were observed.
PCR产物经限制性内切酶消化、序列测定及分析。
最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。
Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the AGAR gel electrophoresis.
利用纯化果胶内切酶对果胶物质的化学结构进行分析比较。
And the chemical structures of pectin substances were compared by the pure endo-polygalacturonase.
首先用限制性内切酶将大的DNA分子切断，产生出特殊的片段。
Large DNA molecules are first dissected with restriction enzymes to produce specific fragments.
在酶法脱墨条件下，单纯的内切酶作用几乎未发现可溶性糖的产生；
Under the de inking conditions, only endoglucanases treatment hardly results in the production of dissolved sugars.
介绍了国内外对脯酰肽内切酶(P EP)抑制剂的最新研究进展。
The development of the study on the PEP inhibitor is introduced in this paper.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
几丁质酶1既是外切酶又是内切酶，而几丁质酶2只表现内切酶活力。
Chitinase 1 showed mixed activities of endo-and exo-chitinase, while chitinase 2 was an endo-chitinase.
根据揭示多态性的限制性内切酶的数量可将产生的突变大多归为点突变。
Based on the number of restriction enzyme detecting RFLPs, most of mutations were attributed to point mutation.
TILLING技术的核心是使用单链核酸内切酶大规模筛选突变体库。
The core technology in TILLING is screening mutant library by using single-strand endonuclease.
目的研究人胃黏膜上皮细胞系无嘌呤无嘧啶核酸内切酶（APE）的表达状况。
Objective To study the expression of APE/Ref-1 in gastric mucosal epithelial cell lines.
方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。
Methods: 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(PCR) technique.
细菌有一种对病毒的防御机制：一种叫做限制性内切酶的化学物质可以切断外源dna。
Bacteria have defences against viruses in the form of chemicals called restriction enzymes, which chop up foreign DNA.
血管活性肠肽只能起到短暂的效果，因为它很快就会被一种叫做中性肽链内切酶的物质分解。
VIP has only a short-lived effect, because it is soon broken down by an enzyme called Neutral Endopeptidase (NEP).
结果显示不论DNA甲基化与否，所有使用受体细胞限制性内切酶的基因组移植都获得成功。
When genome transplantations were performed using the restriction enzyme minus recipient cells, all the genome transplantations worked regardless of if the DNA was methylated or not.
茶花花粉蛋白经过风味酶（外切酶）和中性蛋白酶（内切酶）组成的复合酶水解得到活性肽。
The complex of flavourzyme and neutral protease was used to hydrolyze protein from camellia pollen to obtain bioactive peptides.
结果构建的EBO G87和EBO WT重组载体经内切酶双酶切鉴定及核苷酸序列测定证实。
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
结果表明，CE的分离效能明显高于age，是研究d NA限制性内切酶谱的更有效的检测手段。
Compared with AGE, CE is more outstanding in resolution and detection time, and it can be applied as a more effective means for DNA restriction enzyme pattern analysis.
方法对116例随机收集的1型糖尿病患者用聚合酶链反应-限制性内切酶消化作该点突变的筛选；
Methods The mutation had been screened from 116 unrelated patients with type 1 diabetes mellitus by ApaI digestion of product of polymerase chain reaction amplification.
锌指核酸酶是一种人工制做限制性内切酶，通过将锌指DNA结合区与限制性内切酶的DNA切割区融合获得。
Zinc finger nucleases (ZFNs) are artificial restriction enzymes made by fusing an engineered zinc finger DNA-binding domain to the DNA cleavage domain of a restriction enzyme.
这种技术可在DNA靶标分子的任意位点进行基因敲除、敲入、点突变等操作，无需使用限制性内切酶和连接酶。
The Red mediated recombination can be used to insert, delete or substitute DNA sequences at any desired position on a target molecule without the need for restriction enzymes or DNA ligases.
免疫防御机制演化出许多不同策略，原核生物运用核酸的限制内切酶、抗微生物胜肽及RNA干扰素作为自我防卫。
The evolution of mechanisms for immune defense has resulted in a variety of strategies. Prokaryotes use restriction endonucleases, antimicrobial peptides, and RNA interference for self-protection.
这一技术不需要内切酶消化和连接酶处理，技术操作简单易行，在基因拼接、基因内部突变方面具有良好的应用价值。
The technique didnt need restrict enzymes and ligases, which could be developed as a simple and useful technique in the studies of gene splicing and gene mutation.
目的建立尿中性肽内切酶(NEP)检测的ELISA法，明确用ELISA法检测尿nep在诊断肾小管损伤中的意义。
Objective to set up an ELISA method to measure urinary neutral endopeptidase (NEP) and determine its clinical meaning in diagnosing renal tubular injury.
目的建立尿中性肽内切酶(NEP)检测的ELISA法，明确用ELISA法检测尿nep在诊断肾小管损伤中的意义。
Objective to set up an ELISA method to measure urinary neutral endopeptidase (NEP) and determine its clinical meaning in diagnosing renal tubular injury.
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