在酶法脱墨条件下，单纯的内切酶作用几乎未发现可溶性糖的产生；
Under the de inking conditions, only endoglucanases treatment hardly results in the production of dissolved sugars.
目的建立尿中性肽内切酶(NEP)检测的ELISA法，明确用ELISA法检测尿nep在诊断肾小管损伤中的意义。
Objective to set up an ELISA method to measure urinary neutral endopeptidase (NEP) and determine its clinical meaning in diagnosing renal tubular injury.
方法采用颈部皮瓣悬吊法，经胸骨切迹上切口，鼻内镜辅助下行甲状腺部分切除术7例。
Methods In 7 cases, nasal endoscopy-assisted partial thyroidectomy was accomplished, through skin incision above sternal notch, by using a neck skin lifting method.
从切屑变形力与摩擦力两方面分析了切向磨削力与法向磨削力的比值，大体上应在0.2～0.59范围内。
We have also analysed the ratio between tangential forces and normol forces with respect to both chip formation forces and friction forces. The ratio generally falls within the range of 0.2~0.59.
建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。
The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.
方法：采用PCRSSCP法检测血管网织细胞瘤中VHL基因的突变率及甲基化，敏感限制性内切酶消化法检测血管网织细胞瘤中VHL基因的异常甲基化率。
Methods: The hypermethylation was examined by methyl sensitive restrictive DNA endoenzyme analysis in 34 cases of angioreticuloma and the VHL gene mutations detected by PCR SSCP analysis.
方法：采用PCRSSCP法检测血管网织细胞瘤中VHL基因的突变率及甲基化，敏感限制性内切酶消化法检测血管网织细胞瘤中VHL基因的异常甲基化率。
Methods: The hypermethylation was examined by methyl sensitive restrictive DNA endoenzyme analysis in 34 cases of angioreticuloma and the VHL gene mutations detected by PCR SSCP analysis.
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