与母本扩增带型相比,共扩增出18条特异带,即表现为多态性。
In comparison with female parents, 18 polymorphic bands are specific bands, namely SSR polymorphism.
结果:共扩增出314个条带,其中多态性条带96条,占31%。
Result: 314 bands have emerged, of which 96 are of polymorphism, accounting for 31%.
箭叶橐吾的三个自然居群,总计62个个体,使用17个随机引物,共扩增出128个多态位点。
Three natural population of l. Sagitta, add up to 62 individual, with 17 random primers, 128 polymorphic loci.
结果表明,4对引物共扩增得到272个位点,其中269个多态位点,总的多态位点比例为98.89%。
In total, 272 loci were amplified with 4 primer pairs, of which 269 were polymorphic and the percentage of polymorphic loci was 98.89%.
利用RAPD技术对TBT处理细胞提取的DNA进行分析,在各TBT处理组与对照组相比共扩增出72条差异性片段。
There were 72 differential DNA fragments in the cells disposed to different levels of TBT compared with control by the random amplified polymorphic DNA (RAPD).
其中有88对EST - SSR引物共扩增到101个位点并定位到20条染色体上(染色体4B上没有位点定位)。
A total of 101 EST-SSR loci amplified from 88 primer sets were distributed onto the 20 chromosomes of the reference maps (no marker on chromosome 4b).
结果筛选出的33个随机引物对68个柞蚕品种(系)共扩增出296条DNA带,其中多态性带269条(90.88%)。
ResultA total of 296 DNA fragments were amplified with 33 primers selected from 68 cultivars of A. pernyi, 269 of which (90.88%) were polymorphic.
对12份莲雾资源和2个莲雾近缘种进行了ISSR分析,18条引物共扩增出459个位点,其中多态性位点435个(94.77%)。
ISSR analysis with 18 primers to 12 wax apple(Syzygium samarangense Merr. et Perry)germplasm and 2 closely related species produced 459 unambiguous bands, out of them 435(94.77%) were polymorphic.
所分析的20个随机引物共扩增出了109条带,但是只有引物s303、S4出现4个多态位点,多态性位点所占的比例(P)只有3.7%。
Among 109 loci revealed by 20 RAPD primers, only primer S303 and S4 showed 4 polymorphic loci. The percent of polymorphic loci (p) was only 3.7%.
干预:40只大鼠,于伤后不同时间取水肿区脑组织及正常脑组织:提取组织总rna:用共扩增反转录定量pcr方法测定MMP - 9基因表达水平。
INTERVENTIONS: Tissues of edema area and normal brain of 40 rats were collected in different time after damage: total RNA were abstracted and MMP-9 gene expression was tested by PCR means.
结果表明:17对引物共扩增出154条谱带,其中多态性谱带138条,占89.6%,平均每个引物扩增出8.12条多态性谱带,表现出丰富的多态性。
There were 138 polymorphic alleles, 89.6% out of 154 amplified loci based on 17 selected ISSR primers with average 8.12 alleles each primer that shown a rich genetic diversity among the population.
应用23个随机引物对白粉病菌8个不同生理小种进行RAPD分析,其中11个引物的扩增带型在不同生理小种间出现差异,共扩增出59条谱带,多态性为47.5%。
Differences were found among 8 strains from RAPD analysis. Out of 23 random primers, 11 were polymorphic. Altogether 59 RAPD bands were found and the polymorphic rate was 47.5%.
共筛选80个RAPD随机引物,其中有27个引物能对DNA扩增并能发现其中抗性基因与感性基因的区别。
RAPD were selected in this study used 80 random primers, 27 primer pairs of DNA can be amplified and can be found resistance genes and gene perceptual difference.
应用近等基因池DNA和随机引物,经PCR扩增和共分离分析,建立了二个RAPD片段与抗病基因紧密连锁。
Two PCR amplified fragments were found to be tightly linked to Pi-h-1 (t) by RAPD analysis. of near isogenic pools of DNA from F2 plants.
在51条随机引物中筛选出具有稳定扩增、条带清晰的多态性引物16条,16条引物共检测到132个位点,其中多态位点114个(86.36%)。
RAPD primers were synthesized and 16 primers were selected and used. A total of 132 loci were amplified, of which 114 loci(86.36%) were polymorphic.
在51条随机引物中筛选出具有稳定扩增、条带清晰的多态性引物16条,16条引物共检测到132个位点,其中多态位点114个(86.36%)。
RAPD primers were synthesized and 16 primers were selected and used. A total of 132 loci were amplified, of which 114 loci(86.36%) were polymorphic.
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