方法应用膜片钳全细胞记录模式。
方法膜片钳制技术全细胞记录模式。
METHODS Patch clamp technique with whole cell configuration.
方法：采用常规膜片钳全细胞记录技术。
Method: With conventional Patch Clamp whole-cell recording technique.
方法膜片钳全细胞记录。
全细胞记录模式；
方法在培养的大鼠海马神经元上用膜片钳全细胞记录钾通道电流。
METHODS Voltage gated potassium outward current was recorded using whole cell patch clamp techniques in the cultured rat hippocampal neurons.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
方法采用急性分离的小鼠心室肌细胞，低渗溶液激活容积敏感性氯通道电流，膜片钳全细胞记录法记录电流。
Methods Isolated mouse cardiac ventricular myocytes were subjected to whole-cell patch-clamp to record the hypotonicity activated chloride channel currents.
结果：(1)建立了膜片钳全细胞记录技术平台，获得了高稳定性的正常心室肌细胞全细胞离子通道电流图。
Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法：运用全细胞膜片钳技术记录持续性钠电流，并观察其在缺氧-复氧模型下的变化。
Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.
方法在离体培养的A549细胞上，用膜片钳全细胞模式记录氯通道电流。
Methods the chloride channel currents were recorded in cultured A549 cells by using the whole-cell mode of patch clamp techniques.
方法：采用全细胞膜片钳技术，记录钠通道电流。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
方法用膜片钳记录仪在体外培养的不同时期，记录大鼠海马神经元GABA-A受体离子通道全细胞通道电流、电导的变化。
Methods: The patch clamp was used to record the changes in average whole cell current and the conductance of GABA-A receptor on cultured hippocampal neurons in different developing stages.
采用全细胞膜片钳技术，记录动作电位和上述各离子流。
We used current-clamp technique to record action potentials, and voltage-clamp technique to record ionic currents.
方法：膜片钳全细胞电流记录方法。
Methods: Patch clamp whole cell recording technique was used.
方法应用膜片钳技术全细胞构型从原代培养的螺旋神经元获得电流钳记录。
Methods Current-clamp recordings with the use of the whole cell configuration of the patch-clamp technique were made from the primary cultured spiral ganglion neurons.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
方法应用全细胞膜片钳技术记录乌头碱对大鼠心肌细胞动作电位时程的作用，分别给予奎尼丁、维拉·帕米处理比较二者对动作电位时程的影响。
Methods Effects of quinidine and verapamil on the alteration of action potential duration induced by aconitine were studied by use of the whole cell patch clamp techniques in rat ventricular myocytes.
采用全细胞电压钳记录技术，给予选择性受体阻断剂以分离不同的突触后受体电流。
The recordings of postsynaptic currents were performed by using the whole-cell voltage-clamp method.
以全细胞膜片钳记录单细胞跨膜动作电位和离子电流。
Whole cell patch clamp technique was used to record the action potential and ion currents.
方法：采用高阻抗密封膜片箝全细胞技术，记录分离的豚鼠和家兔心肌单个细胞的钾电流。
METHODS: A tight seal whole-cell patch-clamp technique was used to record ITO, IK, and IK1 in single cells isolated from guinea pig and rabbit hearts.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
MethodsAfter rat hippocampal or cerebral cortical neurons were isolated by pronase e, the IA currents were recorded by whole-cell patch clamp technique.
方法：采用全细胞膜片钳记录法，在新鲜分离的大鼠背根神经节细胞上观察血竭对钠通道电流幅值的影响。
Method: the whole cell patch clamp recording technique was used in this study. Results: blood resin suppressed amplitude of the sodium channel currents in a dose dependent way.
方法：采用全细胞膜片钳技术记录蟾蜍灵激活cne - 2 Z细胞膜电流并分析其电流特征。
METHODS: the technique of whole-cell patch clamp was used to record the chloride currents and to analyze the characteristics of the currents in CNE-2Z cells.
方法：用全细胞膜片箝记录心室肌细胞的L -钙电流。
METHODS: Patch clamp technique (whole cell recording) was used to record L-Ca2 + current in single cardiac myocyte.
用全细胞模式膜片钳技术记录分析血管平滑肌细胞L型电压依赖性钙通道的特性。
The electric physiology feature of L-type calcium channels in the vascular smooth muscle cells was analyzed by patch clamp technique (PCT).
用全细胞模式膜片钳技术记录分析血管平滑肌细胞L型电压依赖性钙通道的特性。
The electric physiology feature of L-type calcium channels in the vascular smooth muscle cells was analyzed by patch clamp technique (PCT).
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