采用不同培养时间的根霉全细胞脂肪酶催化合成己酸乙酯。
Rhizopus chinensis whole-cell lipases of different culture time were used to catalyzesynthesis of ethyl hexanoate.
方法膜片钳制技术全细胞记录模式。
METHODS Patch clamp technique with whole cell configuration.
方法应用膜片钳全细胞记录模式。
方法：膜片钳全细胞电流记录方法。
Methods: Patch clamp whole cell recording technique was used.
方法：采用常规膜片钳全细胞记录技术。
Method: With conventional Patch Clamp whole-cell recording technique.
方法膜片钳全细胞记录。
方法：采用全细胞膜片钳技术，记录钠通道电流。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
全细胞记录模式；
目前，采用全细胞催化等技术工艺有望解决这些难题。
At present, these problems are expected to be solved by using whole-cell catalyst technology.
目的研究艾氏腹水瘤全细胞瘤苗诱导的特异性杀伤活性。
Aim to study the tumor specific cytotoxicity induced by Ehrlich ascites tumor cell vaccine.
全细胞电压膜片钳测定心房细胞的膜电容表示心房细胞的大小。
The atrial cell membrane capacitances were read directly in patch -clamp.
方法在培养的大鼠海马神经元上用膜片钳全细胞记录钾通道电流。
METHODS Voltage gated potassium outward current was recorded using whole cell patch clamp techniques in the cultured rat hippocampal neurons.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
本发明涉及在全细胞催化剂或分离的酶的辅助下从醛生产伯醇的方法。
The invention relates to a method for producing primary alcohols made of aldehydes with the aid of whole cell catalysts or isolated enzymes.
方法在离体培养的A549细胞上，用膜片钳全细胞模式记录氯通道电流。
Methods the chloride channel currents were recorded in cultured A549 cells by using the whole-cell mode of patch clamp techniques.
方法应用膜片钳技术全细胞构型从原代培养的螺旋神经元获得电流钳记录。
Methods Current-clamp recordings with the use of the whole cell configuration of the patch-clamp technique were made from the primary cultured spiral ganglion neurons.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
用膜片钳技术的全细胞模式测定全细胞钠电流并观察应用特布他林对它的影响。
The sodium currents were detected by patch-clamp technique in whole-cell recording mode.
用全细胞模式膜片钳技术记录分析血管平滑肌细胞L型电压依赖性钙通道的特性。
The electric physiology feature of L-type calcium channels in the vascular smooth muscle cells was analyzed by patch clamp technique (PCT).
采用全细胞电压钳记录技术，给予选择性受体阻断剂以分离不同的突触后受体电流。
The recordings of postsynaptic currents were performed by using the whole-cell voltage-clamp method.
重组酵母菌经冻干能有效地实现在非水相中全细胞催化己酸和乙醇酯化合成己酸乙酯。
We constructed a CALB-displaying yeast whole-cell biocatalyst and applied it to esterification from caproic acid and ethanol.
目的研究锌离子对急性分离的大鼠骶髓后连合核神经元甘氨酸激活全细胞电流的调控。
AIM To investigate zinc (Zn2+ ) modulation of glycine (Gly)-activated whole-cell currents in acutely dissociated rat sacral dorsal commissural nucleus (SDCN) neurons.
方法：采用高阻抗密封膜片箝全细胞技术，记录分离的豚鼠和家兔心肌单个细胞的钾电流。
METHODS: A tight seal whole-cell patch-clamp technique was used to record ITO, IK, and IK1 in single cells isolated from guinea pig and rabbit hearts.
目的探讨应用气相色谱技术分析全细胞脂肪酸进行分枝杆菌菌种鉴定方法的准确性和实用性。
Objective to evaluate the accuracy and applicability of species identification method of Mycobacteria by applying gas chromatography analysis of whole-cell fatty acid.
他们需要一个分析的方法来定量在临床病例的AHL水平，所以他们致力于全细胞生物传感器。
They needed an analytical method to quantify AHL levels in clinical samples, so they turned to whole-cell biosensors.
展示酶的酵母细胞作为全细胞催化剂，既具有固定化酶的优点，又有制备简单、成本较低的特点。
An enzyme-displaying yeast as a whole-cell biocatalyst seemed an alternative to immobilized enzyme, due to its low-cost preparation and simple recycle course.
方法：应用全细胞电压箝技术的斜坡脉冲程序，测定离体大鼠心肌细胞准稳态电流-电压关系曲线。
METHODS: The quasi-steady state current-voltage relationship from the isolated rat ventricular myocytes was measured using whole-cell voltage-clamp techniques with a ramp pulse protocol.
发现增殖组织全细胞抽提液中酶比活高于正常分化组织，而且在异常增殖组织中酶比活的增高更为显著。
The specific activity of topoisomerase I was higher in proliferating tissues and much higher in malignant tissues than that in differentiated tissues.
方法采用急性分离的小鼠心室肌细胞，低渗溶液激活容积敏感性氯通道电流，膜片钳全细胞记录法记录电流。
Methods Isolated mouse cardiac ventricular myocytes were subjected to whole-cell patch-clamp to record the hypotonicity activated chloride channel currents.
结果：(1)建立了膜片钳全细胞记录技术平台，获得了高稳定性的正常心室肌细胞全细胞离子通道电流图。
Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.
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