从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
传代培养细胞可在1 ~2周汇合成片。
几次传代培养后，大多数具有遗传稳定性。
Most of them had inheritable stability after several generation.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
考察不同血清对山羊成纤维细胞传代培养的影响。
The effect of different types of serum on the passage of the goat fibroblasts was investigated in this work.
传代培养口腔鳞癌细胞株，制备肿瘤细胞条件培养液；
Oral squamous cells carcinoma cells lines were cultured and passaged, the conditional medium of tumor cells were prepared.
方法大鼠胸主动脉平滑肌细胞传代培养，实验共分六组。
Methods Rat aortic smooth muscle cells were divided into 6 groups randomly.
结论软骨细胞可以通过传代培养获得扩增，但仅限于四代以内细胞。
Conclusions RCC cells can be amplified by passage Culture, but only within the cells of four generations.
硫酸氨基葡萄糖可以抑制这一趋势，促进传代培养的细胞增殖能力。
GS can inhibit the tendency and strengthened the proliferating ability of chondrocytes in serial subcultivation.
本文探讨了连续传代培养中营养对昆虫病原线虫繁殖力及产量的影响。
The effects of nutrition on fecundity and yield of nematode in continued culture were evaluated.
贴壁细胞传代培养10代后接近融合，细胞扩增了约10 0 0倍。
The adherent cells approached confluence after 10 passages, and was increased in number about 1000-fold.
方法：采用酶消化法传代培养乳sd大鼠颅盖骨细胞并建立缺氧模型。
Methods: the osteoblasts obtained from the calvaria of neonatal SD rats were cultured by method of enzyme digestion in vitro.
方法：原位传代培养羊水细胞并制备染色体，G显带分析核型，产后随访。
Methods:Amniotic fluid cells were cultured in situ and their karyotypes were analyzed by G band, followed them after delivery.
方法：用胰酶消化法获取人胎儿视网膜色素上皮细胞进行原代及传代培养。
Methods: The fetal RPE cells were cultured with trypsin enzyme digesting technique and passaged in vitro.
背景：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
BACKGROUND: Key point for subculture of human embryonic stem cells is to inhibit spontaneous differentiation and make sure totipotency of cells.
目的：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
AIM: the key of the human embryonic stem cell culture is to guarantee the totipotency and inhibit spontaneous differentiation.
兔角膜上皮和内皮细胞组织块原代培养，消化接种于人胚肺饲细胞瓶传代培养。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage.
通过组织块培养法，获得了传代培养的鲁西黄牛耳皮肤成纤维细胞和上皮细胞。
Luxi cattle-ear skin fibroblast and epithelial cells were derived by fragment of tissue.
在DMEM培养基中进行了原代及传代培养，对其中的成纤维细胞进行分离纯化。
The isolated cells were cultured in DMEM medium and PDL fibroblasts were purified in subculture.
结果：原代培养的成纤维细胞特征典型，并通过传代培养得到了纯化的成纤维细胞。
Results: The cultured cells showed typical shape and features of fibroblast cell, which were successfully dissociated and purified.
取大鼠肱三头肌，采用二步消化法分离骨骼肌卫星细胞，体外进行原代培养和传代培养。
Rat triceps brachii muscle was acquired to separate skeletal muscle satellite cells with the two-step method of collegenase-1 and trypsin and were cultured and subcultured in vitro.
在暴露于空气后不同的时间段将菌株分别经改良布氏肉汤及哥伦比亚平板进行传代培养。
Culturing the Hp strains which had already been exposed to air for a different time with the improved broth medium and Columbia plate respectively.
方法分别自3周龄大耳白兔关节软骨和半月板分离软骨细胞，行单层传代培养和离心管培养。
Methods Chondrocytes isolated from articular cartilage and meniscus of rabbits aged 3 weeks were respectively passaged in monolayer and cultured in centrifuge tube.
实验选取清洁级SD新生鼠10只，无菌条件下分离脑组织，克隆传代培养脑源性神经干细胞。
Ten newly born SD rats of clean grade were selected to isolate the brain tissue under sterile condition and clone brain-derived NSCs.
另外研究血管内皮生长因子(VEGF)和传代培养对细胞分化、扩增动力学和细胞凋亡的影响。
In addition, we tested the effect of vessel endothelial growth factor (VEGF) and passage on cell differentiation, expansion kinetics and apoptosis.
结果原代培养的细胞ALP染色阳性区域可以达90%以上，传代培养后细胞阳性率为100%。
Results the area positive for ALP staining is no less than 90% in the primary culture and almost 100% in the subculture.
目的：研究甲肝病毒在细胞内快速连续传代培养病毒增殖情况，探索疫苗生产工艺改进的可能性。
Objective: to study the proliferation of HAV which were cultured rapidly and consecutively in cells, and to investigate the possibility of improving the production process of inactivated HAV vaccine.
经6次传代培养后，其生物量和菌丝多糖产量较稳定，且明显超过原始菌株，表明其遗传稳定性良好。
After continuous cultivation for 6 generations, the biology yield and the polysaccharide yield were steady and obviously exceeded the original strain. It showed a good genetic stability.
细胞传代培养和冻存6个月，7td1MC 5细胞对IL - 6的反应性和依赖性均无明显变化。
No obvious variation of reactivity and dependence of 7td1 MC5 cell on IL-6 was observed after 6 months Subculture and Cryostorage.
结果流式细胞术证明，MSC具有间充质细胞的特征。原代及传代培养显示，胎儿骨髓msc具有活跃增殖的能力。
Results Flow cytometry analysis showed that fetal bone marrow derived MSC exhibited the characteristics of mesenchymal cells, MSC had an active proliferative ability in primary and passage cultures.
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