研究了用中国仓鼠卵巢细胞(CHO)生产促红细胞生成素(EPO)的配方。
The medium formulation have been studied to culture CHO cells producing erythropoietin(EPO).
目的:通过悬浮适应,使中国仓鼠卵巢细胞(CHO细胞)获得悬浮生长的特性,并可在悬浮培养条件下较快地生长。
Objective: Through suspension adaptation, to endow the Chinese hamster ovary cells (CHO cells) with suspension growth characteristics and rapid growth ability.
方法:以高表达人CD59的中国仓鼠卵巢细胞(CHO)为靶细胞,采用竞争结合试验,对噬菌体随机12肽库进行5轮亲和筛选。
METHODS: Using CHO cells which high express human CD59 as target cells, the phage 12 peptide library was screened for 5 rounds by competitive binding test.
目的:在中国仓鼠卵巢(CHO)细胞上稳定转染咪唑啉1受体。
Objective: to stably transfect imidazoline-1 receptor (I 1r) in Chinese hamster ovary (CHO) cells.
目的:在中国仓鼠卵巢(CHO)细胞上稳定转染咪唑啉1受体。
Objective: to stably transfect imidazoline-1 receptor (I 1r) in Chinese hamster ovary (CHO) cells.
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