目的探讨白念珠菌耐药基因CDR1基因上游调控序列对氟康唑耐药的影响。
Objective To appraise the relationship between fluconazole resistance and expression of the CDR1 gene upstream regulation sequence.
方法：检索并分析釉原蛋白基因的上游调控序列。
METHODS: The upstream regulation sequences of amelogenin gene were retrieved and analyzed.
方法：将基因内翻译起始序列合成后克隆到上游含有起始密码和无起始密码的报告质粒中，研究表达水平的差异。
Methods:One of the three internal translation initiation region(TIR) was chemically synthesized and cloned into the report plasmid, and the expression of the report gene was studied.
重组体用克隆位点上游和下游的T7和T3启动子序列为测序引物，用自动测序仪测序鉴定克隆的正确性。
The recombinant was sequenced by automatic sequencer with promoters T7 in upstream and T3 in downstream as sequencing primers.
因此选择对基因的表达具有重要功能的外显子1和其上游的调控区及下游外显子内含子交界区序列通过直接测序法进行SNP筛查，并进一步分析多态位点与病窦的关联性。
Therefore the other aim of our study is to discover SNPs of HCN4 exon 1, including the regulatory sequence and exon-intron boundary of it, which plays an importable role of channel function.
加下划线的核苷酸分别是这个读码框中上游的第一个终止密码和两个已经认定的多核苷酸序列。星号标记的就是终止密码。
The underlined nucleotides are the first upstream in-frame stop codon and the two putative polyadenylation signals, respectively. The asterisk indicates the stop codon.
加下划线的核苷酸分别是这个读码框中上游的第一个终止密码和两个已经认定的多核苷酸序列。星号标记的就是终止密码。
The underlined nucleotides are the first upstream in-frame stop codon and the two putative polyadenylation signals, respectively. The asterisk indicates the stop codon.
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