首先从实验对象的外周血中提取DNA,pcr扩增il-4的第二个内含子中的一段TG重复序列，然后用8%的聚丙烯酰胺凝胶电泳检测这段基因的多态性。
DNA were extracted from the peripheral blood, then TG sequence in second intron of IL - 4 was amplified by PCR, the polymorphism of that segment were then detected through 8% polyacrylamide gel.
首先从实验对象的外周血中提取DNA,pcr扩增il-4的第二个内含子中的一段TG重复序列，然后用8%的聚丙烯酰胺凝胶电泳检测这段基因的多态性。
DNA were extracted from the peripheral blood, then TG sequence in second intron of IL - 4 was amplified by PCR, the polymorphism of that segment were then detected through 8% polyacrylamide gel.
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