采用套式聚合酶链反应方法对合并TTV感染情况进行回顾性研究。
TTV infection in the patients was retrospectively analyzed with nested PCR detection of TTV DNA in serum.
方法用半巢式聚合酶链反应方法检测72例非甲—庚型肝炎患者血清中ttv - DNA。
Methods TTV-DNA in serum of 72 patients with hepatitis Non-A to G were detected by Hn-PCR.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
依据聚合酶链反应(PCR)和酶联免疫吸附检测(ELISA)的方法目前被应用于主要食源性致病菌的检测。
Polymerase chain reaction-based (PCR-based) and enzyme-linked immunoabsorbent assay (ELISA) methods are now applied in the detection of major food-borne pathogens.
方法：采用聚合酶链反应(PCR)技术，检测了64例肺结核患者的外周血与痰标本结核杆菌dna。
Methods: Polymerase chain reaction (PCR) was adopted in detection of DNA of mycobacterium tuberculosis from peripheral blood and sputum in 64 tuberculosis patients.
目的建立应用任意引物聚合酶链反应进行真菌菌型鉴定的方法。
Objective To establish the method to identify fungus using the arbitrarily primed polymerase chain reaction (APPCR).
目的建立套式聚合酶链反应（PCR）加限制性酶切分析方法检测人巨细胞病毒（HCMV）。
Objectives To establish an nested (polymerase chain reaction) PCR analytic method with restrictive enzyme to detect human cytomegalovirus (HCMV) rapidly in clinics.
方法聚合酶链反应。
方法应用实时监测聚合酶链反应检测这三种受体基因在雌激素受体阳性和雌激素受体阴性乳腺癌细胞系中的表达。
Methods These three genes were quantified by real-time quantification polymerase chain reaction (PCR) in er positive and er negative breast cancer cell lines.
目的探讨克隆聚合酶链反应(PCR)扩增产物的简便方法。
Objective To explore simple method for cloning the products amplified with polymerase chain reaction (PCR).
方法运用序列特异性引物聚合酶链反应技术，检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
用逆转录-聚合酶链反应(RT - PCR)方法检测主动外排泵基因CDR1和CDR2的表达水平。
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of active efflux gene CDR1 and CDR2.
方法采用逆转录聚合酶链反应(RT -PCR)检测84例静脉毒瘾者血浆标本。
Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR).
方法采用多对型特异性引物-聚合酶链反应检测160例慢性乙型肝炎患者血清HBV基因型；
Methods: Serum samples from 160 cases with chronic HBV infection were collected and tested for HBV genotypes by type-specific primers.
方法对医院临床分离的8株耐亚胺培南铜绿假单胞菌进行随机引物聚合酶链反应（PCR）扩增，扩增产物进行电泳和聚类分析。
METHODS The genes of imipenem-resistant P. aeruginosa were amplified by RAPD assay in 8 clinical isolates and PCR products were analyzed by agarose gel electrophoresis and cluster analysis.
聚合酶链反应(PCR)可作为一种快速检测并鉴别马疱疹病毒1型(EHV 1)和马疱疹病毒4型(EHV 4)的诊断方法。
A rapid method for detection and identification of equine herpesvirus 1 (EHV-1) and equine herpesvirus 4 (EHV-4) was developed using polymerase chain reaction (PCR).
方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
方法采用聚合酶链反应-限制性片段长度多态性分析(PCR - RFLP)检测136例HBVDNA阳性的上海籍HBV感染者的基因型。
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法应用聚合酶链反应限制性片段长度多态性分析方法(PCR -RFLP)对40例口腔鳞癌组织中apc基因的杂合缺失(LOH)进行检测。
Methods We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) to examine 40 oral squamous cell carcinomas for loss of heterozygosity (LOH) at APC.
方法：采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本，通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法：应用半巢式聚合酶链反应技术检测113例孕产妇血清及其新生儿脐血的TTV—DNA。
Methods: TTV-DNA of 113 cases of parturients' blood serum and neonatal umbilical blood were detected by semi-nested polymerase chain reaction.
方法采用逆转录—聚合酶链反应(RT -PCR)方法检测40例患者的肺癌组织ttf - 1基因的表达。
Methods 40 patients with lung cancer were studied to detect TTF-1 gene by using reverse transcription-polymerase chain reaction assay (RT-PCR).
方法胆红素氧化酶突变体i 402g和C457S通过聚合酶链反应获得，并经氨基酸序列测定加以证实。
Methods The BO mutants I402G and C457S were obtained by site-directed mutagenesis and confirmed by amino acid sequence analysis.
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
方法：采用序列特异性引物聚合酶链反应的方法对汉族72例uc患者和314例正常对照者HLA - DRB1基因分型。
Methods: By using polymerase chain reaction-sequence specific primers, frequency of HLADRB1 alleles in 72 patients with UC and 314 healthy controls were detected.
方法应用荧光定量聚合酶链反应(FQ - PCR)对尖锐湿疣高危人群分泌物标本进行HPV - DNA分型检测。
Methods the secretion specimen coming from the high risk patients with CA were examined with fluorescent quantitative polymerase chain reaction (FQ-PCR) for genotype HPV-DNA.
方法：采用逆转录-聚合酶链反应(RT - PCR)检测48例胃癌组织及30例正常组织中vegf基因的表达。
Methods: The expression of VEGF gene in 48 samples of human gastric carcinomas and 30 samples of corresponding normal tissue specimens was detected by RT-PCR.
方法采用聚合酶链反应技术（PCR）检测沙眼衣原体和解脲支原体的DNA。
Methods DNA of CT and UU was detected with polymerase chain reaction(PCR) technique.
方法采用单管四重聚合酶链反应(PCR)技术。
Methods Single barrel four-seat PCR technique was performed.
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