在植物组织培养过程中，继代培养是其中的重要环节，而长期继代则是种质资源离体保存的必要手段。
Subculture is an important link in the process of the tissue culture and the long-term subculture is the essential method for conservation in vitro of germplasm.
结果：人脐静脉内皮细胞继代培养36代。
Results:Human umbilical vein endothelial cells were subcultivated in vitro over 36 th generation.
经3～4次继代培养即可得到悬浮的单细胞。
Suspension single cells were obtained after successive subculturing of 3 or 4 generations.
长期继代培养的柑橘胚性愈伤组织由胚性细胞和非胚性细胞组成。
In Citrus, the embryogenic calli consisted of embryogenic and non-embryogenic cells.
经过两年多(15个世代)的继代培养，建立了党参体细胞无性系。
Somatic clones were established after 15 generations of subculture for more than two years.
利用继代培养愈伤组织进行细胞悬浮培养并检测黄酮的生物合成量。
The biosynthesis amount of flavonoids was assayed in the callus suspension culture.
结果表明：CC培养基是绝大多数水稻愈伤组织的最适诱导与继代培养基；
The main results were as following: For most rice CC medium was the best for both callus initiation and subculture.
从水稻成熟胚诱导出的愈伤组织经继代培养后可以产生大量的胚性愈伤组织。
Many embryogenic calli were produced by subculture of calli induced from mature embryo of rice.
在继代培养基中添加山梨醇能改善愈伤组织的生长状态，降低愈伤组织的褐化。
Adding sorbitol to the subculture medium could improve the growing state of the calli and decrease the percentage of Browning calli.
本文首次于国内报道原代和继代培养猪冠状动脉平滑肌细胞的光、电镜结构特征。
This study reports the features of porcine coronary artery smooth muscle cells in the primary culture and subculture with light and electron microscopes for the first time in China.
但幼胚诱导的愈伤组织经固体继代培养和液体悬浮培养后，都没有胚状体的分化。
But all the calli induced by immature embryos did not differentiate the embryoids through solid and liquid subculture.
自然生长、初代培养和继代培养的马铃薯再生体的水溶性蛋白之间存在着一些差异。
There were some differences in water soluble proteins for the natural, primary cultured and subcultured potatoes.
结果表明，在连续继代培养的第74~83代复合系的菌种组成没有变化，非常稳定。
The composition of bacteria strain of 74~83 generations of continuous subculture in composite system did not change, with special stability of the composition.
香石竹红色品种茎尖试管苗继代培养玻璃化现象是香石竹脱毒试管苗生产的一个主要障碍。
Vitrification is the main obstacle in subculture of carnation plantlets in vitro, which were induced from shoot tips.
DON毒素对小麦细胞培养物的影响与毒素浓度、外植体来源和继代培养时间等因素有关；
The effect of DON toxin on cultured wheat cells was related to the toxin concentration, explants and subculture duration.
将海岛棉茎尖分生组织经过3～6周的诱导、继代培养生长后，可以形成足够量的再生植株。
Sufficient plantlets of regeneration were produced within 3~6 weeks induced and culture from apical meristems.
本实验室以继代培养的丛生芽块为材料，经过连续加盐筛选、后代选育，获得了一批耐盐株系。
The multiple bud clumps subcultured were a series of salt filtration and offspring breeding selection. Then salt-tolerant plantlets were regenerated and T_2 lines were obtained.
将抗药突变体在无药培养基上继代培养9代后，其抗性倍数逐渐下降，抗药性可能不能稳定遗传。
The resistant rate of resistant mutants gradually decreased after 9 generations of subcultures in the absence of fungicides and the resistance obtained could be unstable.
变异细胞系经无盐继代培养后仍具有较高的游离脯氨酸积累能力，表明变异细胞系具有稳定的耐盐性。
The variant cell lines still have high ability of accumulating free proline after cultivation on normal medium, which indicated the variant cell lines have steady salt tolerance.
在保种培养基基础上，改变植物激素6-BA浓度配制不同的培养基，对麝香百合继代苗进行继代培养；
This paper studied the effects of different levels of 6-BA on the growth of tissue culture seedlings of Lilium longiflorum.
大麦成熟胚的初代愈伤组织是水质、柔软的非胚性愈伤组织，在继代培养中能以一定频率出现胚性愈伤组织。
The primary callus induced from barley mature embryos is soft, watery and nonembryogenic, but after subculture it can produce compact embryogenic callus with a certain frequency.
变叶木的品种不同 ，其愈伤组织的生长及再生植株能力 ，在继代培养中后期表现出明显的品种特异性 。
For different varieties of variegated leafcroton, the growing capacity of callus and plant regeneration displayed evident varietal specificities in late stage of subcultures.
采用均匀正交设计UL9（34）分析了6-BA，IBA，蔗糖不同浓度及其组合对长柄扁桃继代培养的影响。
The effects of 6-BA, IBA and sucrose in different concentrations and combinations on subculture of Amygdalus pedunculata were analyzed by using uniform orthogonal design UL9(34).
取继代培养30 d的铁皮石斛类原球茎作接种材料，以N6为基本培养基，加入植物激素，并以真菌提取物作诱导子，对类原球茎和愈伤组织进行诱导培养。
Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants, N6 was used as the basic culture with phytohormone added, and fungal extracts as the elicitor.
在继代过程中，适当提高2,4 - D浓度可抑制愈伤的分化，但为防止高浓度2,4 - D对愈伤的伤害，分化趋势一旦控制应立即转入低浓度2,4 - D的继代培养基中。
In callus subculture, increase in 2, 4-d content kept the callus from differentiation, though, high concentration of 2, 4-d did harm to the callus.
在继代过程中，适当提高2,4 - D浓度可抑制愈伤的分化，但为防止高浓度2,4 - D对愈伤的伤害，分化趋势一旦控制应立即转入低浓度2,4 - D的继代培养基中。
In callus subculture, increase in 2, 4-d content kept the callus from differentiation, though, high concentration of 2, 4-d did harm to the callus.
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