细胞传代20次后，仍生长良好。
考察不同血清对山羊成纤维细胞传代培养的影响。
The effect of different types of serum on the passage of the goat fibroblasts was investigated in this work.
背景：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
BACKGROUND: Key point for subculture of human embryonic stem cells is to inhibit spontaneous differentiation and make sure totipotency of cells.
结果用该法培养细胞传代生长快，细胞纯度达98%以上，足以满足各种细胞试验需求。
Results VSMC was grown well and fast, the purity is more than 98% which can meet various cell tests.
这一发现表明在ES细胞传代时的培养条件(已被认识到)能影响人类ES细胞的发育特征。
The discovery implies that culture conditions during es cell generation - which have yet to be identified - can influence the developmental properties of human es cells.
细胞传代培养和冻存6个月，7td1MC 5细胞对IL - 6的反应性和依赖性均无明显变化。
No obvious variation of reactivity and dependence of 7td1 MC5 cell on IL-6 was observed after 6 months Subculture and Cryostorage.
据报道，ES细胞目前已有在体外培养两年，连续传代达450次以上，而胚胎生殖干细胞传代次数最多可达70~80次。
According to reports, ESC culture in vitro was more than two years and attained 450 serial passages. Embryonic germline ESC attained 70~80 passages at most.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养，保留贴壁细胞传代，分析脂肪间充质干细胞的纯度，观察细胞生长情况，检测其增殖活性、细胞周期。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
结果：原代培养的RPE细胞含大量色素呈多角形，传代的细胞透明呈梭形。
Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape.
早期传代细胞染色体数出现非整倍体和非稳定性畸变，同时有两个标记染色体；
There were aneuploid and instable structure changes in chromosomes, and two marker chromosomes at the same time.
目的探讨血管内皮细胞在传代过程中的衰老过程及机制。
Objective To study the process of aging and its potential mechanisms during passage of vascular endothelial cells.
本文就胚胎质量和胚胎类型分化抑制物培养基传代时间和消化液等方面对胚胎干细胞分离克隆的影响进行了讨论。
Influences of embryo quality and type differentiation stayer media passage and digestion fluid on embryonic cell separation and cloning were discussed respectively.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法分别自3周龄大耳白兔关节软骨和半月板分离软骨细胞，行单层传代培养和离心管培养。
Methods Chondrocytes isolated from articular cartilage and meniscus of rabbits aged 3 weeks were respectively passaged in monolayer and cultured in centrifuge tube.
方法用狗肾传代(MDCK)细胞和鸡胚双腔法进行流感病毒分离。
Methods The influenza virus isolation was performed by MDCK cells and embryonated eggs.
作者引进并建立了大鼠小肠上皮细胞(IEC—6)的培养传代和活性检测的实验方法；且对几种影响因素进行了探讨。
A culture method and assay for the rat small intestinal epithelial cell (IEC-6) line have been established and factors that influence the results of the culture have been investigated.
方法：用胰酶消化法培养兔rpe细胞并传代。
Methods: The rabbit RPE cells were cultured with trypsin enzyme-digesting technique and passaged in vitro.
即使在连续传至128代的CSFV39 - PK 15传代细胞中，CSFV仍持续存在：呈免疫荧光抗体反应阳性和RT - PCR检测阳性。
Even in the 128th CSFV39-PK15 passage cells, we still could detect the existence of CSFV39. Both immunofluorescence antibody test and RT-PCR assay showed positive results.
结论：所建立的RT _PCR方法可自病毒感染的小鼠脑组织和传代细胞中检测WEE病毒的基因组rna。
Conclusions: a specific RT_PCR assay was established for detecting the genomic RNA of WEE virus from infected Suckling mice and culture cells.
观察原代和传代细胞的生长情况。
The primary and passages of retinal progenitors were observed.
方法将人肝门部胆管癌标本进行细胞培养，传代。
Methods The fresh human hilar cholangiocarcinoma specimen was cultured.
小鼠骨髓内皮细胞经过长期培养后，已转化为内皮细胞株，已传代20次，其增殖能力没有减退。
Murine bone marrow endothelial cell line could be established after long time culture and there is no significant reduction in their proliferation potency after 20 passages.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
结论：利用多次差速传代可从混合培养的原代细胞中获得纯化的牙囊细胞。
Conclusion: Dental follicle cells can be purified by several differential passages from the mixed primarily cultured cells.
采用无血清液体培养基、DMEM培养基和MEM培养基进行PK15细胞的培养，观察细胞增殖及传代效果，确定无血清液体培养基的营养功能。
PK15 cells were cultured with free-blood serum media, DMEM media and MEM media in other to observe the effect of cell proliferation, cell transfer and define the ability of nutrition.
方法：采用细胞培养的方法建立稳定传代的胰腺癌hs 766t细胞系，体外培养人脐带静脉内皮细胞。
Methods: We built the pancreatic cancer cell group HS 766t and human endothelium cell in the umbilical vein by the way of cell culture in vitro.
另外研究血管内皮生长因子(VEGF)和传代培养对细胞分化、扩增动力学和细胞凋亡的影响。
In addition, we tested the effect of vessel endothelial growth factor (VEGF) and passage on cell differentiation, expansion kinetics and apoptosis.
自成年新西兰兔股骨转子部取得骨髓基质细胞进行传代培养，所得骨髓基质细胞再与钙磷陶瓷载体复合培养制成复合人工骨。
Bone marrow cells from New Zealand white rabbits were cultured to obtain stromal cells, which were then cultured with the carriers to make the artificial composite bone graft.
兔角膜上皮和内皮细胞组织块原代培养，消化接种于人胚肺饲细胞瓶传代培养。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage.
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