目的：对野生肉苁蓉及其组织细胞培养物进行润肠通便、抗疲劳功能的比较研究。
Objective:To compare the Runchang purge and anti-fatigue effects of Cistanche deserticola and the tissue culture of Cistanche deserticola.
方法：体外试验采用组织细胞培养法，观察不同浓度药物对流感病毒FM1病毒株致细胞病变（CPE）的作用；
Method: The method of cell and tissue culture was utilized to carry out an observation on the cytopathy effect(CPE) led by influenza virus FM1 with the aerosol of different concentrations in vitro.
应用PCR方法检测组织细胞培养物中的病毒增殖和增殖滴度，测定了PCV2病毒收获时间，试验表明PCV2病毒在PK15组织培养可连续传代，增殖病毒。
PCR method was applied to detect the proliferation condition and titer of PCV2 and to determine the harvest time of PCV2. The results showed that PCV2 could proliferate and passage on PK15 cells.
应用PCR方法检测组织细胞培养物中的病毒增殖和增殖滴度，测定了PCV2病毒收获时间，试验表明PCV2病毒在PK15组织培养可连续传代，增殖病毒。
PCR method was applied to detect the proliferation condition and titer of PCV2 and to determine the harvest time of PCV2. The results showed that PCV2 could proliferate and passage on PK15 cells.
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