而一个数组芯片可以包含目的基因的许多片断。
An array is created that consists of lots of fragments from genes of interest.
目的基因序列在宿主细胞成功表达。
Objective gene sequence was expressed successfully in host cells.
注：下划线部分为目的基因同源臂序列。
Note: the underlined part is the homologous sequence of the target knockout gene.
目的基因的重组与稳定性。
结论：逆转录pcr技术是获得目的基因的有效方法。
Conclusion: Reversible transcription PCR technique is an effective method for obtaining definite gene.
获取理想的目的基因PCR反应的综合参数比十分重要。
Synthetic parameters of the PCR are very important to obtain ideal target gene.
而转染鼠成纤维细胞NIH3T3，未见目的基因表达。
The target gene couldn't express in NIH3T3 cells and blank control.
方法采用了胚胎移植，目的基因检测，胚胎冷冻等技术。
Methods Techniques such as embryonic transplantation, genotype and embryo cryopreservation were applied.
方法：以引物互为模板的聚合酶链反应合成目的基因片段。
Methods: The PCR technique in which the primers were used as template each other was adopted to obtain the foreign DNAs for Cloning.
有限数目的基因和全部生存率和肝移植后无癌生存率相联系。
A limited number of genes were related to overall survival and cancer-free survival after LT.
目的：优化哺乳动物细胞表达系统，提高目的基因的表达效率。
Objective: to increase the expression level of foreign gene by the optimization of the expressional regulatory elements in the cassette of interesting gene.
GUS染色证明外源目的基因在玉米细胞和组织中得到了表达。
GUS assay indicated that foreign gene had expressed in maize cell and calli.
我们的研究选择了轮状病毒SA11株VP4基因作为目的基因。
We choose rotavirus SA11 strain VP4 gene as the target gene.
目的：建立一个可诱导表达目的基因的细胞系以研究新基因的功能。
Objective: to establish a stable cell line that can be induced to express a target gene.
方法：采取目的基因改造及载体构建的方法，以期构建高效表达载体。
Methods: The method of modifying aim gene and constructing vector was adopted to develop a vector with high efficacy.
经鉴定证实目的基因发生了特异转座和获得的病毒为特异的重组病毒。
It was approved that the gene transposition and specific virus recombination were performed from the constructed plasmids.
将目的基因导入小梁组织使房水排出阻力增加是一种理想的造模方法。
Overexpression of certain gene(s)in trabecular meshwork to increase the resistance to aqueous humor outflow would be an ideal method to create an animal model of glaucoma.
PCR分子检测与转化的标记基因和外源目的基因抗性三者极有规律性。
The results of PCR molecular detection, marker genes and foreign target-gene with insect-resistance were quite regular.
目的比较脂质体和糖化多聚赖氨酸对目的基因肝脏靶向定位效果的影响。
Aim To compare the effects of liposome and glyco-poly-L-lysine (G-PLL) on target uptake and gene expression of liver by intravenous injection.
目的建立一种灵敏度高、重现性好的检测混合样品中微量目的基因的方法。
Objective To develop a high sensitivity and reproducible method for rapid DNA analysis even when using picogram sample in a complex background.
结果表明，经20代以上的回交，除目的基因外，均为轮回亲本的遗传组成。
The result indicated that the genetic construction of congenic inbred strains was similar with the parents through more than the 20th generation back cross mating except for destination gene.
利用PCR反应和T载体可以从少量的标本中，高效获得高纯度的目的基因。
High-pure gene fragments could be obtained from little amount of sample through PCR and T-vector.
该技术主要包括：目的基因和靶细胞的制备、样品的灌注以及对细胞的显微注射。
The main procedures were: to prepare the target genes and cells, to fill the sample into the glass capillary injector and to proceed microinjection.
前言： 目的：探讨和尝试一种较为迅速确定最佳退火温度的方法来扩增目的基因。
Objective:To explore a method for quicker identification of the optimal annealing temperature in PCR.
VHL基因可作为临床诊断指标，并可望成为肾透明细胞癌基因治疗的重要目的基因。
The VHL gene may be useful as a marker gene for the diagnosis of RCC and as a target gene for molecular therapy.
筛选及鉴定与药物成瘾记忆相关的目的基因是研究的第一步，也是本课题的主要内容。
The first step is to screen and identify genes correlated closely to the memory of drug addiction, which is also the main content of my study.
目的：从基因组dna获取编码脑源性神经营养因子基因，并对目的基因进行序列测定。
Objective: to amplify the gene for human brain - derived neurotrophin factor (BDNF) directly from human genomic DNA and to assure it by sequencing.
结论应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
Conclusions Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR.
结论应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
Conclusions Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR.
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