猪瘟是由猪瘟病毒引起的高度传染性猪病。
Classical swine fever (CSF), a highly contagious disease of swine, was caused by classical swine fever virus (CSFV).
猪瘟感染确诊的主要依据是对CSFV的实验室检测。
目的：获取猪瘟病毒内蒙流行株的E2基因并构建融合基因。
Objective To obtain the E2 genes from Neimeng strain of classical swine fever virus and construct fusion genes.
猪瘟是由猪瘟病毒引起猪的一种高度接触性和致死性传染病。
Classical swine fever virus (CSFV) is the causative agent of classical swine fever, a highly contagious and often fatal disease of domestic pigs.
寻找一种更具针对性、更为可靠的仔猪猪瘟抗体水平检测方法。
The present experiment was conducted to develop a direct and reliable detection method for classical swine fever (CSF) antibody level of weaned piglets.
这种方法此前曾被用于防控非洲和亚洲的牛瘟，以及南美洲的猪瘟。
The method has previously been used to fight rinderpest in Africa and Asia and hog cholera in South America.
目的：为了探讨一种比较适合我省的猪瘟合理免疫程序，进行本实验。
Purpose: in order to discuss a kind of reasonable immunity procedure for Hog Cholera that suited for our province, we did this experiment.
实验结果证实 LAMP技术可成功应用 于猪瘟病毒核酸序列之检测。
The results from the above experiments suggested that LAPM technique can be successfully applied in the detection of classical swine fever virus.
对猪瘟RT PCR诊断方法与兔体交叉试验诊断方法的相关性进行了研究。
Relationship between RT-PCR and rabbit-cross test for detection of classical swine fever virus (CSFV) was studied in this experiment.
猪瘟病毒(CSFV)能在多种体外培养细胞中增殖，却不使细胞产生病变(CPE)。
Classical Swine Fever Virus (CSFV) can multiplicated in many kinds of culture cells, and its multiplication does not cause cytopathic effects (CPE) on their host cells.
本发明属卫生检验领域，涉及一种用于检测猪瘟病毒的LAMP试剂盒及其建立方法和应用。
The invention belongs to the field of sanitary examination, and relates to a LAMP kit for testing classical swine fever virus and an establishing method and an application thereof.
猪瘟病毒(CSFV)可在多种动物的组织细胞中复制，但一般不产生致细胞病变效应(CPE)。
CSFV could replicate and proliferate in multiple animals 'tissue cells, but not cause the cytopathic effect (CPE) in general condition.
本发明公开了一种用于检测非洲猪瘟病毒抗体的竞争ELISA试剂盒及其用途，属于生物技术领域。
The invention discloses a competitive ELISA kit for detecting african swine fever virus antibody and the purposes thereof, which belong to the biological technical field.
猪瘟的暴发可以导致养猪业的巨大经济损失且影响国际贸易，被世界动物卫生组织列为报告疾病名录。
CSF outbreaks can result in large economic losses in pig production and affect international trade and therefore CSF is a notifiable disease to the World Organization for Animal Health (OIE).
采用IHA实验方法监测猪瘟灭活疫苗免疫的猪抗体产生水平，并对仔猪母源抗体的消长规律进行了研究。
Antibodies in swine induced by classical swine fever inactivated vaccines were evaluated by IHA test, and the maternal antibodies in piglets were studied.
本文报导了某猪场30 ~50日龄哺乳仔猪暴发猪瘟的流行情况、症状、病理变化以及实验室诊断结果。
This article reported the prevalence condition, symptom, the change of the pathology, and the result of laboratory diagnosis for the outbreak of 30 ~ 50 days aged piglets' HC.
可以将CSFV野毒感染猪迅速从免疫猪群中筛查出来并予以清除，有利于猪瘟的净化和无猪瘟疫区的建立；
By using the assay, pigs infected with wild-type CSFV can be identified and removed from the vaccinated population, which will help to establish a CSF-free swineherd.
本研究旨在构建稳定表达T7RNA聚合酶的猪睾丸细胞系(ST细胞系)，用于猪瘟病毒等的反向遗传操作。
This study was aimed to establish a ST-based cell line stably expressing the T7 RNA polymerase, which can be used in the reverse genetic manipulation of CSFV and other swine RNA viruses.
试剂盒采用原核表达的重组P30蛋白作为包被抗原，依据竞争ELISA原理检测猪血清中非洲猪瘟病毒的抗体。
The kit adopts prokaryotic expression recombinant P30 protein as coating antigen, and detects the African swine fever virus antibody in pig serum according to a competitive ELISA principle.
“非洲猪瘟病毒向高加索地区的蔓延给家畜健康带来极大的威胁并有可能导致出现严重局面，”粮农组织首席兽医官约瑟夫·多梅内奇说。
"The spread of the African Swine Fever virus to the Caucasus region poses a very serious animal health risk and could lead to a dramatic situation," said FAO Chief Veterinary Officer Joseph Domenech.
猪瘟是由猪瘟病毒（CSFV）引起的一种猪高度接触性传染病，临床上可引起猪的最急性、急性、亚急性、慢性和非典型病症。
Classical swine fever (CSF) is an important disease in pigs caused by the CSF virus (CSFV), which belongs to the genus Pestivirus within the family Flaviviridae.
荷兰在典型的猪瘟发生了一个多月之后才发现，诊断前已经有39头猪被感染。然而，不当的控制和根除措施是造成这类疾病流行的原因，控制和根除它们更困难，成本也高得多。
Conversely, inappropriate control and eradication measures are at the root of such endemic situations, which are much more difficult, and infinitely more costly, to keep under control or eradicate.
以120份疑似猪瘟病料为试验材料，使用RT-PCR核酸检测与ELISA抗原检测2种方法，分别用这2种方法检测疑似病料，并且比较这2种方法在猪瘟病料检测中的实际差别。
In order to analyze the differences between RT-PCR and ELISA antigen detection methods in classical swine fever virus detection, 120 clinical samples were detected by the two methods.
以120份疑似猪瘟病料为试验材料，使用RT-PCR核酸检测与ELISA抗原检测2种方法，分别用这2种方法检测疑似病料，并且比较这2种方法在猪瘟病料检测中的实际差别。
In order to analyze the differences between RT-PCR and ELISA antigen detection methods in classical swine fever virus detection, 120 clinical samples were detected by the two methods.
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